Optimizing Carbon and Nitrogen for Protease Production in Endophytic Bacteria from Mandeh, Pesisir Selatan

Rima Dwitaviani, Anthoni Agustien, Akmal Djamaan

Abstract


The protease enzyme possesses the ability to hydrolyze proteins into peptide bonds and amino acids, serving as a biocatalyst that accelerates reactions, thus holding potential across various industries. The aim of this research is to determine the influence of carbon and nitrogen sources, as well as their concentrations, on the optimum protease activity of a collection of bacterial isolates from Sonneratia sp. in the Mandeh area, Pesisir Selatan. The research employed an experimental methodology, and protease activity was assessed using the Takami method. The results of this study indicated that bacterial isolates EUA-131 and EUA-135 exhibited optimum activity with the addition of 1% glucose and 1% NaNO3, while isolate EUA-136 showed optimum activity with the addition of 1.5% maltose and 1% KNO3.


Keywords


Sonneratia sp., bacterials, takami method, protease

Full Text:

PDF

References


Yücel, Y. Optimization of immobilization conditions of Thermomyces lanuginosus lipase on olive pomace powder using response surface methodology. Biocatalysis and Agricultural Biotechnology, 1(1), 39–44. (2012). https://doi.org/10.1016/j.bcab.2011.08.009

Zanphorlin, L. M., Facchini, F. D. A., Vasconcelos, F., Bonugli-Santos, R. C., Rodrigues, A., Sette, L. D., Gomes, E., & Bonilla-Rodriguez, G. O. Production, partial characterization, and immobilization in alginate beads of an alkaline protease from a new thermophilic fungus Myceliophthora sp. Journal of Microbiology, 48(3), 331–336. (2010). https://doi.org/10.1007/s12275-010-9269-8

Annonymous. Mordor Intelligence "Protease Market Size & Share Analysis - Growth Trends & Forecasts (2023 - 2028)". 2023.

Gupta, R., Beg, Q., & Lorenz, P. Bacterial alkaline proteases: Molecular approaches and industrial applications. Applied Microbiology and Biotechnology, 59(1), 15–32. (2002). https://doi.org/10.1007/s00253-002-0975-y

Moo-Young, M., & Chisti, Y. Biochemical engineering in biotechnology (Technical Report). Pure and Applied Chemistry, 66(1), 117–136. (1994).https://doi.org/10.1351/pac199466010117

Sierecka, J. K. Purification and partial characterization of a neutral protease from a virulent strain of Bacillus cereus. International Journal of Biochemistry and Cell Biology, 30(5), 579–595. (1998). https://doi.org/10.1016/S1357-2725(98)00007-7

Cooper, Geoffrey M. "The central role of enzymes as biological catalysts." Sinauer Associates, 2000.

Hossack, Euan J., Florence J. Hardy, and Anthony P. Green. "Building enzymes through design and evolution." ACS Catalysis 13.19 (2023): 12436-12444.

Patel, R. K., Dodia, M. S., Joshi, R. H., & Singh, S. P. Production of extracellular halo-alkaline protease from a newly isolated Haloalkaliphilic Bacillus sp. isolated from seawater in Western India. World Journal of Microbiology and Biotechnology, 22(4), 375–382. (2006). https://doi.org/10.1007/s11274-005-9044-x

Ray, A. K., Bairagi, A., Sarkar Ghosh, K., & Sen, S. K. Optimization of fermentation conditions for cellulase production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut. Acta Ichthyologica et Piscatoria, 37(1), 47–53. (2007). https://doi.org/10.3750/AIP2007.37.1.07

Masruroh, Isna Firli, et al. "Screening of Factors Influencing Keratinase Fermentation from Bacillus Haynesii BK1H using The Plackett-Burman Design (PBD)." E3S Web of Conferences. Vol. 481. EDP Sciences, 2024.

Snyman, C., Theron, L., & Divol, B. Understanding the regulation of extracellular protease gene expression in fungi: a key step towards their biotechnological applications. Applied Microbiology and Biotechnology, 103(14), 5517–5532. (2019). https://doi.org/10.1007/s00253-019-09902-z

Sun, Yu, et al. "Extracellular protease production regulated by nitrogen and carbon sources in Trichoderma reesei." Journal of Basic Microbiology 61.2 (2021): 122-132.

Köhl, Jürgen, Rogier Kolnaar, and Willem J. Ravensberg. "Mode of action of microbial biological control agents against plant diseases: relevance beyond efficacy." Frontiers in plant science 10 (2019): 454982.

Masi, C., Vivek, P., Sowmya, V., Sindhuja, V., & Parthasarathi, N. Production and process optimization of protease using various bacterial species – A review. International Journal of ChemTech Research, 6(9), 4268–4275. (2014).

Agyei, D., & Danquah, M. K. Carbohydrate utilization affects Lactobacillus delbrueckii subsp. Lactis 313 cell-enveloped-associated proteinase production. Biotechnology and Bioprocess Engineering, 17(4), 787–794. (2012). https://doi.org/10.1007/s12257-012-0106-2

Sharma, K. M., Kumar, R., Panwar, S., & Kumar, A. Microbial alkaline proteases: Optimization of production parameters and their properties. Journal of Genetic Engineering and Biotechnology, 15(1), 115–126. (2017). https://doi.org/10.1016/j.jgeb.2017.02.001

Ishizuka, H., Hanamura, A., Kunimura, T., & Aiba, H. A lowered concentration of Kingston-Smith, A. H., Bollard, A. L., & Minchin, F. R. (2005). Stress-induced changes in protease composition are determined by nitrogen supply in non-nodulating white clover. Journal of Experimental Botany, 56(412), 745–753. (1993).https://doi.org/10.1093/jxb/eri049

Jindal, S., Gupta, S., Gupta, R., & Kumari, A. Nitrate addition enhances the production of protease and amylase by Bacillus amyloliquefaciens ASH. Brazilian Journal of Microbiology, 47(1): 1-10. (2016).

Xu, X., Liu, G., Liu, J., Lyu, M., Wang, F., Xing, Y., Meng, H., Li, M., Jiang, Y., Tian, G., Zhu, Z., Jiang, Y., & Ge, S. Potassium Alleviated High Nitrogen-Induced Apple Growth Inhibition by Regulating Photosynthetic Nitrogen Allocation and Enhancing Nitrogen Utilization Capacity. Horticultural Plant Journal. (2023). https://doi.org/10.1016/j.hpj.2023.04.003

Bhunia, Biswanath, Bikram Basak, and Apurba Dey. "A review on production of serine alkaline protease by Bacillus spp." Journal of Biochemical Technology 3.4 (2012): 448-457.

Abusham, R. A., Rahman, R. N. Z. R. A., Salleh, A., & Basri, M. Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand. Microbial Cell Factories, 8, 1–9. . (2009). https://doi.org/10.1186/1475-2859-8-20

Colla, L. M., Reinehr, C. O., Manfredini, P. G., Farina Cavanhi, V. A., & Vieira Costa, J. A. Simultaneous production of proteases and antioxidant biopeptides by solid-state fermentation. Sustainable Food Technology, 874–885. (2023). https://doi.org/10.1039/d3fb00077j

Sárraga, C., Gil, M., Arnau, J., Monfort, J. M., & Cussó, R. Effect of curing salt and phosphate on the activity of porcine muscle proteases. Meat Science, 25(4), 241–249. (1989). https://doi.org/10.1016/0309-1740(89)90042-9




DOI: http://dx.doi.org/10.52155/ijpsat.v44.2.6179

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Rima Dwitaviani

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.