Effect of Low Protein Diet Treatment for Six Weeks on The Brain Histopathological and Cognitive Function in The Wistar Rat

Rita Maliza, Allimuddin Tofrizal, Fadillah Fadillah


Malnutrition is a condition characterized by inadequate or excessive consumption of nutrients, resulting in decreased cognitive abilities, metabolic disorders, learning disabilities, growth retardation, a lowered immune system, and the risk of death. The prevalence of malnutrition in Indonesia was still relatively high. This research aims to identify the effect of low-diet protein treatment on the histopathology of the cerebral cortex, hippocampus, and cognitive function in Wistar rats. This study included two groups of male Wistar rats: the normal and low protein (12%) groups. Morphometric analysis was used to assess the growth. Cognitive tests included the Y-Maze, Novel Object Recognition Test (NORT), and Water Maze by Morris (MWM) tests, and histopathological examination of the brain sections was conducted on the cerebral cortex and hippocampus by hematoxylin and eosin stains. This study found that rats fed a 12% protein diet showed significant differences in body weight parameters, proportion of degenerated cells, and histopathological features of the cerebral cortex and hippocampus compared to the normal group fed a 20% protein diet, and this condition also affected the cognitive function of rats fed a low protein diet. The 6-week treatment with a 12% protein concentration left the rats malnourished.


Malnutrition; Body Weight; Cognitive Function; Low Protein Diet

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DOI: http://dx.doi.org/10.52155/ijpsat.v44.1.6134


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