Species Assignment to A Polyhydroxybutyrate-Producing Microbacterium Isolate from Egypt

Heba Mohamed Saad, H. ElSheshtawy, O. A. Elshehaby, Y.A. Osman

Abstract


Poly (3-hydroxybutyrate) is a natural polymer belonging to the family of polyhydroxyalkanoates, which accumulates in a wide variety of bacterial strains as reserve food material under imbalanced growth condition. Poly (3-hydroxybutyrate) is a biodegradable alternative to traditional petrochemical plastics such as polypropylene and polyethylene. The Microbacterium paraoxydans (WA81) is a local isolate producing large amounts of Poly (3-hydroxybutyrate) which was confirmed by Sudan black B stain and transmission electron microscopy. Moreover, this isolated was further characterized by bacteriological and molecular methods such as sensitivity to antibiotics, plasmid profile and MALDI-TOF-MS. This local isolate contained a 10kb single plasmid and was identified as Microbacterium paraoxydans DSM 15461 using MALDI- TOF –MS technique

Keywords


PHB , PHA, Microbacterium , MALDI-TOF-MS, biodegradable plastic.

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DOI: http://dx.doi.org/10.52155/ijpsat.v24.2.2672

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