A New Method for Differentiating Mesenchymal Stem Cells toward Neural Lineage

Mahmoud Elhussiny Salama, Mohamed Elshahat Ebaid, Tamer Mohamed Roshdy, Mohamed Salama, Mohamed Sobh

Abstract


AIM: To investigate and find an efficient method for inducing mesenchymal stem cells (MSCs) into neural differentiation. METHODS: MSCs from rat bone marrow were purified using adhesiveness property of stem cells, then characterized with flowcytometer. The isolated cells seeded in neural induction medium for 4 days, Morphology and immunocytochemistry were used to identify the neural differentiation grade. RESULTS: MSCs exhibited neural network after differentiation, instead of homogenous fibroblast-like morphology before differentiation. Flowcytometric analysis confirmed the identity of mesenchymal stem cells. more than 80 percent of all population were stained positive for Tau protein.

 CONCLUSION: Bone marrow MSCs have ectodermal differentiation capabilities. MSCs can differentiate into functional neuron-like cells, and may be used as a treatment for neurodegenerative diseases.

 

 


Keywords


Keywords: stem cell, neuron, differentiation, Tau protein, Neurodegenerative disease.

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DOI: http://dx.doi.org/10.52155/ijpsat.v21.2.1969

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