Isolation and Screening of Cellulolytic Bacteria from Mangrove Waters in Mandeh, West Sumatera Province, Indonesia

Dwiky Rahmadi Aswan, Feskaharny Alamsjah, Anthoni Agustien

Abstract


Microbial communities in mangrove ecosystems produce cellulase enzymes, essential for breaking down cellulose, which are used in various applications. The objective of the research is to isolate and purify cellulolytics from the mangrove waters on the Mandeh coast, West Sumatera Province, Indonesia. Thirteen sites were used for this study, and water samples were chosen at random. The findings demonstrated that 16 isolates exhibited cellulolytic activity, and 38 isolates that grew in nutrient agar were created by the purified bacteria. The common bacteria found have a high colony elevation, an undulating colony edge, an uneven colony form, and cream pigmentation. The most prevalent form of bacteria is the bacilli cell, and 16 of the 38 isolates exhibited cellulase activity after inoculation. The cellulolytic index (CI) ranged from 0.36 to 4.82, and the highest CI was PUA-18 (4.82), followed by PUA-38 (4.6), PUA-28 (3.74), and PUA-21 (3.61).

Keywords


bacteria; cellulase; cellulolytic isolation; mangrove; screening

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References


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DOI: http://dx.doi.org/10.52155/ijpsat.v45.2.6295

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