Evaluation Of Probiotic Potential Of Bacillus Subtilis FFOS For Application In Relevant Industry

Ujunwa Felicia Nwachukwu

Abstract


Probiotics are known to improve the health of the host. Several microorganisms including Bacillus species have been reported to have probiotic potentials. Therefore the paper aimed to assess Bacillus subtilis FFOS for probiotic potential for its application in relevant industry.  To determine probiotic potentials, several test including antagonistic activity, bile resistance, hemolytic activity and acid tolerance assays were carried out. The organism, Bacillus subtilis FFOS was applied in agriculture (poultry and farming). During application in poultry, nutrient broth (3%) containing the test organism was supplemented in the chicken feed for 12weeks and the feed without supplement served as control. The growth of each chicken was determined every week using a weighing balance. The 2-bean seeds were planted into 3 bottles containing loamy soil respectively. Plant in bottle 1 (Sample A) was grown with nutrient broth containing Bacillus subtilis FFOS; plant in bottle 2 (Sample B) was grown with nutrient broth alone and bottle 3(Sample C) grown with water alone and their growth rates were monitored daily until 12days. The obtained result revealed antimicrobial potential of Bacillus subtilis FFOS against all the test pathogens (Escherichia coli, Salmonella sp. and Staphylococcus aureus) but more with Escherichia coli (8-10mm). Bacillus subtilis FFOS resist bile up to 7% concentration (40x103CFU/mL), showed no hemolysis and withstand acidic conditions (pH 3) up to 97.5 %. The assayed organism significantly enhanced the chicken growth (4.01kg/12wks)(p<0.05). The broth supplemented with Bacillus subtilis FFOS enhanced the plant growth more than that without supplement and the one grown with water alone. The plant grown with water alone have higher growth than that grown with broth alone. The obtained result revealed Bacillus subtilis FFOS; a probiotics,  good chicken growth enhancer and plant growth promoter. Hence it can be used as probiotics and supplement in chicken feed and plant growth.

 


Keywords


Evaluation, Bacillus subtilis FFOS, probiotics, application, supplement.

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References


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DOI: http://dx.doi.org/10.52155/ijpsat.v45.1.6284

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