Immunogenicity Of Boiled Pork Extracts And The Protein Band From SDSPAGE Of Fresh Pork Extracts

Deshinta Putri Mbajeng Kinenda, Djoko Kisworo, Sulaiman Ngongu Depamede

Abstract


The aim of this study was to analyze the immunogenicity of boiled pork extract antigens and the isolate SDSPAGE protein bands of fresh pork. The antigens were injected subcutaneously, repeatedly at two-week intervals into two local rabbits. Antibodies were isolated two weeks after the last booster. Analysis of the immune response was carried out using the indirect immunoblot assay. The results of this study showed that boiled pork antigens were more immunogenic than SDSPAGE bands of fresh pork. The specific activity of the antibodies obtained was about six times higher than the antibodies from rabbits vaccinated with SDSPAGE bands of fresh pork extract.  Whether the antibodies obtained can be used to develop meat adulteration authentication test kits still needs to be investigated.

Keywords


Antibody; Boiled Pork Extracts; Immunoblot; Meat Adulteration; SDSPAGE Bands.

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DOI: http://dx.doi.org/10.52155/ijpsat.v44.2.6210

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