Antimicrobial and Antioxidants Activity Test of Fresh Extract and Decoction Extract of Areca Plant Roots (Areca catechu Linn.) Against Test Microbes

Nurmiati Nurmiati, periadnadi periadnadi, sri wijaya


Research on the Antimicrobial and Antioxidant Activities of Several Areca (Areca catechu Linn.) Root Extracts which was conducted at the Microbiology Laboratory, Andalas University in April - June 2023. This study aims to determine the antimicrobial activity of several areca root extract treatments, determine the most effective extraction of the tested microbes and determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration(MBC) of areca root against the tested microbes, as well as the antioxidant activity and polyphenols of areca root extract. The method used in this study is the nested pattern experimental method. The results showed that the inhibition zone of areca root extract had a significantly different effect on S. aureus bacteria, but did not have a significantly different effect on E. coli bacteria. and the fungus C. albicans. The MIC of areca root extract against E. coli and S. aureus bacteria is 3.12% with MBC on E. coli 12.5% and S. aureus 25%, while the MIC for C. albicans is 25% and there is no MIC. Antioxidant value of fresh extract, fresh decoction and dry decoction of betel roots respectively with IC50 values of 22.37 μg/ml, 16.79 μg/ml and 84.01 μg/ml for very strong activity category and total polyphenols obtained from fresh extract , fresh decoction and dry decoction were 30.66 mgGAE/ml, 33.16 mgGAE/ml, and 19.89 mgGAE/ml, respectively.


Antimicrobial, Antioxidant, Areca catechu L, Extraction, Polyphenols

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