An Overview: Effect of Plant Growth Regulatory on Orchid Propagation through The Thin Cell Layer technique

Media Media, Zozy Aneloi Noli, M. Idris

Abstract


Thin cell layer (TCL) is a micropropagation method using thin-sized explants. A TCL can be prepared from any explant source and the thichness of explant less than 5 mm. TCL is more efficient in producing total plantlet output than conventional in vitro methods. TCL have been applied to the in vitro culture of orchids, field, vegetable crops and medicinal plants The successful in vitro orchid propagation is influenced by many factors, such as plant genotype and media composition. Additions of plant growth regulator (PGR) in media culture is essential factor. The formation of complete plant depent on concentration and type of plant growth regulator. TCL explants require growth regulators to form an embryonic callus and zygotic embryos. Explant in medium without supplemented of growth regulators resulted in browning and failed to grow. The most commonly plant growth regulator in tissue culture are thidiazuron (TDZ), 6-benzylaminopurine (BAP) 2,4 dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), and meta-Topolin. Recently, microalgae can also be used as an alternative source of hormones to increase plant growth through in vitro culture techniques

Keywords


in vitro; micropopagation; PGRs;TCL

Full Text:

PDF

References


S. Biswas and D. R. Singh, “A manual on orchid education (issue august)”. ICAR-National Research Center for Orchids, East Sikkim, India, 2019.

S. Hariyanto, I.A. Pratiwi, and E.S.W. Utami, “Seed morphometry of native Indonesian orchids in the genus Dendrobium,” Scientifica, vol. 2(1), pp.1-14, 2020, doi: 10.1155/20203986369.

M. F. Fay, M. Feustel, C. Newlands, and G. Gebauer, “Inferring the mycorrhizal status of introduced plants of Cypripedium calceolus (orchidaceae) in northern England using stable isotope analysis,” Botanical Journal Linnean Society.,vol. 186, pp.587–590, 2018.

N. Setiari, A. Purwantoro, S. Moeljopawiro and E. Semiarti, “Micropropagation of Dendrobium phalaenopsis orchid through overexpression of embryo gene AtRKD4,” Agrivita journal of Agricultural Science, vol 40(2), pp. 284–294, 2018, doi: 10.17503/agrivita.v40i2.1690.

F. Yulianti, H. Arisah, and D. Agisimanto, “Pengujian stabilitas genetik planlet Citrumelo hasil TCL dari kultur in vitro dengan menggunakan teknik sekuen berulang,” Jurnal Hortikultura, vol. 27(2), pp. 165-172, 2017. doi: 21082/jhort.v27n2.2017.p165-172.

M.M, Hossain, R. Kant, V. Pham, B. Winarto, S. Zeng, and J.A. Taxiera da Silva, “The application of biotechnology to orchids,” Critical Reviews in Plant Scienes, vol. 32(2), pp. 69–139, 2013, doi: 10.1080/07352689.2012. 715984.

D. Agisimanto, “Thin Cell Layer mempercepat pembuatan populasi genotip unggul hortikultura,” Iptek Hortikultura, vol. 11, pp. 67-72, 2015.

M. Ekmekcigil, M. Bayraktar, O. Akkus and A. Gurel. 2019, “High frequency protocorm like bodies and shoot regeneration through a combination of thin cell layer and RITA temporary immersion bioreactor in Cattleya forbesii Lindl,” Plant Cell, Tissue and Organ Culture, vol 136(4), pp. 451-464, 2019, doi: 10.1007/s11240-018-1526-2.

N. Cetin, N. B Guler and A. Gurel, “In vitro regeneration potential of thin cell layer explants of Lentisk (Pistacia lentiscus var. Chia) plant,” Bilecik Seyh Edebali Universitasi Fen Bilimleri Dergis., vol. 8(2), pp. 960-977, 2021, doi:10.35193/bseufbd.947888.

G.f. Jing, W.N. Aqilla, and Z.A.R.S. Subramaniam. “The effect of thin cell layer in Vanilla planifolia in vitro culture,” Current Botany, vol. 5, pp. 22-25, 2014.

L.R.P. Gomes, C.D.R.B. Franceschi, and L.L.F Ribas, “Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture,” Acta Scientiarum Biological Sciences, vol. 37(2), pp. 143–149, 2015 doi: 10.4025/actascibiolsci.v37i2 .27276.

D.L.Ferreira, E.D.C. Smidt, and L.L.F. Ribas, “Efficient micropropagation of Epidendrum secundum Jacq. from leaves and protocorms,” African Journal of Biotechnology, vol. 14(13), pp. 1122-1128, 2015, doi:10.5897/AJB2015.14467.

N. Wattanapan, C. Nualsri, and U. Meesawat, “In vitro propagation through transverse thin cell layer (tTCL) culture system of Lady’s Slipper Orchid: Paphiopedilum callosum var. sublaeve,” Songklanakarin Journal of Science and Technology, vol. 40(2), pp. 306-313, 2018, doi:10.14456/ sjst-psu.2018.48.

S. Parthibhan, M.V. Rao, J.A. Teixeira Da Silva, and T.S. Kumar, “Somatic embryogenesis from stem thin cell layers of Dendrobium aqueum,” Biologia Plantarum, vol. 62(3), pp. 439-450, 2018, doi:10.1007/s10535-018-0769-4.

P.Bhattacharyya, P. Paul, S. Kumaria, and P. Tandon, “Transverse thin cell layer (t-TCL) mediated improvised micropropagation protocol for endangered medicinal orchid Dendrobium aphyllum Roxb: an integrated phytomolecular approach,” Acta Physiologiae Plantarum, vol. 40(8), pp. 137-150, 2018, doi: 10.1007/s11738-018-2703-y.

K. Chin Lo, J.A. Gansau, C.H. Shih, and C.Y. Kao, “Shoot development through modified transverse thin cell layer (tTCL) culture of Phalaenopsis Hybrid protocorms,” Horticulturae, vol. 8(3), pp. 206-215, 2022, doi: 10.3390/HORTICULTURAE8030206.

J.A. Teixeira da Silva, and J. Dobranszki, “Plant thin cell layers: a 40-year celebration,” Journal Plant Growth Regulator, vol. 32(4), pp. 922-943, 2013, doi: 10.1007/s00344-013-9336-6.

R. Ghahremani, S.D. Daylami, M. Mirmasoumi, N. Askari, and K. Vahdati, “Refining a protocol for somatic embryogenesis and plant regeneration of Phalaenopsis amabilis cv. jinan from mature tissues,” Turkish Journal of Agriculture and Forestry, vol. 45(3), pp. 356-364, 2021, doi: 10. 3906/tar-2004-107.

C.P. Lindley, R.B. Arniputri, L.A. Soliah, and O. Cahyono, “Effects of organic additives and naphthalene acetid acid (NAA) application on the in vitro growth of black orchid hybrid Coelogyne pandurata Lindley. Bulgarian Journal of Agriculture Science; vol. 23(6), pp. 951–957, 2017.

A.R. Nurana, G. Wijana, and R. Dwiyani, “Pengaruh 2-iP dan NAA terhadap pertumbuhan planlet anggrek Dendrobium hibrida pada tahap subkultur,” Agrotrop, vol. 7 (2), pp. 139 – 146, 2017.

N. Rangsayatorn, “Micropropagation of Dendrobium draconis Rchb. f. from thin cross-section culture,” Scientia Horticulturae, vol. 122, pp. 662–665, 2009.

B. Bose, S. Kumaria, H. Choudhury, and P.Tandon, “Insights into nuclear DNA content, hydrogen peroxide and antioxidative enzyme activities during transverse thin cell layer organogenesis and ex vitro acclimatization of Malaxis wallichii, a threatened medicinal orchid. Physiol Mol Biol Plants, vol. 23, pp. 955-968, 2017, doi: 10.1007/s12298-017-0474-3

J. R. Corbellini, L. L. F. Ribas, A. R.D. Maia, D. O. Corrêa, M. D. Noseda, R. M. Suzuki, E. Amano, “Effect of microalgae Messastrum gracile and Chlorella vulgaris on the in vitro propagation of orchid Cattleya labiata.,” Journal of Applied Phycology, vol.32(6), pp. 1-15, 2020, doi: 10.1007/s10811-020-02251-9

Kasli, “Upaya perbanyakan tanaman krisan (Crysanthemum sp.) secara in vitro,” Jerami, vol. 2(3), pp. 121-125, 2009.

G.E. Schaller, I.H. Street, and J.J. Kieber, “Cytokinin and the cell cycle,” Current opinion in plant biology, vol. 21, pp. 7 – 15, 2014.

A. Gentile, A. Frattarelli, P. Nota, E. Condello, and E. Caboni, “The aromatic cytokinin meta-topolin promotes in vitro propagation, shoot quality and micrografting in Corylus colurna L.,” Plant Cell Tissue Organ Culture, vol. 128, pp. 693-703, 2017.

A. Poeaim, W. Kunwanlop, W. Boonmee, and P. Laipasu, “Effects of 6-benzylaminopurine and meta-topoline on micropropagation of Dendrobium chrysanthum Wall. ex. Lindl.,” International Journal of Agricultural Technology, vol. 18(5), pp. 2185-2192, 2022.

K. Nowakowska, and A. Pacholczak, “Comparison of the Effect of Meta-Topolin and Benzyladenine during Daphne mezereum L. Micropropagation, 10(12), pp 1994-2008 2020. doi:10.3390/agronomy10121994.

A. Tanaka, and R. Tanaka, “The biochemistry, physiology, and evolution of the chlorophyll cycle. In Metabolism, Structure and Function of Plant Tetrapyrroles: Introduction, Microbial and Eukaryotic Chlorophyll Synthesis and Catabolism; Grimm, B.B.T.-A., Ed.; Academic Press: Cambridge, MA, USA, 2019; Volume 90, pp. 183–212. ISBN 0065-2296.

A. Ali, A. Asbahi, M. Ali, A. Maqtari, and K.M. Naji, “ABA biosynthesis defective mutants reduce some free amino acids accumulation under drought stress in tomato leaves in comparison with Arabidopsis plants tissues,” Journal of Stress Physiology and Biochemistry, vol. 8, pp. 179–192, 2012.

C.A. Huetteman, and J.E. Preece, “Thidiazuron: apotent cytokinin for woody plant tissue culture,” Plant Cell, Tissue and Organ Culture, vol. 33, pp. 105- 119. 1993.

P.K. Saxena, K.A. Malik, and R. Gill, ”Induction by thidiazuron of somatic embryogenesis in intact seedling of peanut,” Planta, vol. 187, pp. 421-424, 1992.

S. Baghel, and Y.K. Bansal, “Thidiazuron promotes in vitro plant regeneration and phytochemical screening of Guizotia abyssinica Cass. - a multi purpose oil crop” World Journal of Pharmacy and Phamaceutical Sciences, vol. 4(1), pp. 1193 – 1217, 2015.

M.P. Jones, J. Cao, R. O’Brien, S.J. Murch, and P.K. Saxena, “The mode of action of thidiazuron: auxins, indoleamines, and ionchannels in the regeneration of Echinacea purpurea L.,” Plant Cell Reports, vol. 26, pp. 1481-1490, 2007.

H.A. Bilal, B. Guo., and Z. Amir, “Thidiazuron: a multi-dimensional plantgrowth regulator,” African Journal of Biotechnology, vol. 10(45), pp. 8984–9000, 2011.

E.F.C. Ningrum, I.N. Rosyidi, R.R. Puspasari, dan E. Semiarti, “Perkembangan awal protocorm anggrek Phalaenopsis amabilis secara invitro setelah penambahan zat pengatur tumbuh α -naphtalene acetic acid dan thidiazuron,” Biosfera, vol. 34(1), pp. 9 – 14, 2017.

M. Syamsiah, A.A. Imansyah, H.K. Suprapti and D.S. Badriah, “Respon multiplikasi anggrek bulan (Phalaenopsis sp.) terhadap penambahan beberapa konsentrasi bap (benzyl amino purine) pada media in vitro,” Agroscience, vol. 10(2), pp.148-159, 2020.

I.A. Rineksane, S.S. Nafi'ah, and S.S. Dewi, “The combination of rice water and BAP enhances the multiplication of Grammatophyllum speciosum,” Journal of Agro Science, vol. 6(2), pp. 92–99, 2018, doi: 10.18196/pt. 2018.085.92-99

D.P.S. Hendaryono, and A. Wijayani, “Teknik Kultur Jaringan,“ Yogyakarta: Kanisius, 1994.

E.F. George, and P.D. Sherrington, “Plant propagation by tis¬sue culture handbook and directory of comercial laboratories,” Basingtoke, England, 1984.

R.A. Nurhanifah, A. Supriyatna A. Adawiyah, “Induksi tunas anggrek (Dendrobium Sp) Var. Kumala menggunakan BAP dan air kelapa secara in vitro,” Gunung Djati Conference Series, vol. 6, pp. 156-162, 2021.

K.N. Qomariah, E. Semiarti and A. W. Hasbullah, “Propagasi Dendrobium stratiotes Rchb.f. dengan benziladenin secara in vitro,” Agrosaintifika, vol. 1, pp. 14–21, 2018.

L. Taiz, and E. Zeiger, “Plant Physiology 5th Edition,” USA: Sinauer Associates Inc., Sunderland, Massachusetts, USA, 2012.

N.S. Pereira, B.R.R. Ferreira, E.M. de Carvalho, and C.R. Damiani, “Application of Chlorella sorokiniana (Chlorophyceae) as supplement and/or an alternative medium for the in vitro cultivation of Schomburgkia crispa (Orchidaceae),” Journal of Applied Phycology, vol. 30, pp. 2347–2358, 2018.

H. Mazur, A. Konop, R. Synak, “Indole-3-acetic acid in the culture medium of two axenic green microalgae,” Journal of Applied Phycology, vol. 13, pp. 35–42, 2001.




DOI: http://dx.doi.org/10.52155/ijpsat.v39.2.5506

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Zozy Aneloi Noli

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.