The Influence of Bitter (Andrographis Paniculata Ness.) Extract to Reproductive Performance of Mice (Mus Musculus L. Swiss Webster)

Wendikal Abadi, Ramadhan Sumarmin

Abstract


Sambiloto (AndrographispaniculataNess) is one of the folk remedies that are expected to be a herbal anti fertility. Chemically this plant contain diterpenoid lactones do. On the components of diterpenoid lactones do contains andrografolid which is the main active substances from the plant that acts as an anti fertility. Andrografolid function to prevent cell division (cytokinesis). this research aims to know the influence of the giving of the extract (Andrographispaniculata Ness.) against the appearance of the reproduction of Mice (Musmusculus l. Switzerland Webster). This research is a research experiment with a random Design method complete (RAL) that use animal testing mice are females (Musmusculus l.) by as much as 4 treatments and 4 replicates. The treatment given is the extract of Sambiloto with various doses, IE: A is the control treatment, without given extract, sambiloto treatments 1, 2 and 3 is the treatment given the extract of sambiloto orally (gavage) for 24 days with each dose is 1. 0.2 g/head/day, 2. 0.4 g/head/day, 3. 0.6 g/head/day. The parameters observed were stem weight, number of corpus luteum, implantation, resorpsi, dead fetus, fetus alive and the average weight of the fetus. The data are tested with the parametric ANOVA (analysis of Variance) and then in the advanced test BNT on drajat α 5%. The results showed that the extract of sambiloto ((Andrographispaniculata Ness.) effect on the amount of the foetus is born where every real different treatment when compared to controls. as well as the number of implantation and the number of corpus luteum that has decreased when compared with controls. Stem weight added on P1, P2 and P3 are experiencing the decline in when compared with controls. Foetus alive decline on P1, P2 and P3 if compared to the control. Then it can be inferred that the extract of sambiloto given orally during the 24 days led to a decrease in the number of corpus luteum, decline  number of fetus born and decreased the number of implantation.


Keywords


Sambiloto, Andrografolid, Reproductive Performance

Full Text:

PDF

References


BadanPusat Statistik (BPS). 2013. Proyeksi Penduduk Indonesia 2010-2035. Jakarta

Campbell, N. A, J. B. Reece dan L. G. Mitchell. 2014. BiologiEdisiKelimaJilid II. Jakarta: Erlangga

.[3]Lu, Frank. C. 1995. Toksikologi Dasar, Asas Organ Sasaran dan Penilaian Resiko. Penerjemah, Edi Nugroho. Jakarta: UI Press.

Margaretta. 2013. Pengaruh Ekstrak Sambiloto (Andrographis paniculata Ness.) Terhadap Kualitas Spermatozoa Epididimis Mencit (Mus musculus L.) Swiss Webster (Skripsi).Prodi pendidikan Biologi (STKIP) PGRI Sumbar. Padang.

Muna, L., O.P.Astirin., dan Sugiyarto. 2011. Uji Teratogenik Pandanus conoideus Varietas Buah Kuning Terhadap Perkembangan Embrio Tikus Putih (Rattus norvegicus). Nusantara Bioscience.2. pp 126-134.

Mohan, M., S. Khanam.,and B.G. Shivananda. 2013. “Optimization of Microwave Assisted Extraction of Andrographolide from Andrographis paniculata and its Comparison with Refluxation Extraction Method”. Journal of Pharmacognosy and Phytochemistry. Vol 2(1): 342-348.

Nuratmi, B., Adjirni, D.L. Paramita. 1996.Beberapa Penelitian Farmakologi Sambiloto (Andrographis paniculata Ness). Warta Tumbuhan Obat Indonesia 3: 1-24.

Palupi, J. 2008. “Pengaruh Pemberian Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) Per Oral Terhadap Folikulogenesis Ovarium Mencit (Mus musculus L.)”. Jurnal Kesehatan, Vol 6 (2).

Rahayu, M dan F. M. Setiowati.2007. “Etnobotani Sambiloto, Pemanfaatannya Sebagai Bahan Obat Tradisional.” Pusat Penelitian dan Pengembangan Biologi-LIPI Bogor. Vol 3 (1): 29.

Ritter, E,J. 1977. Altered biosynthesys in: Wilson, J.G and F.C. Fraser (eds) Hand Book of Teratology. Vol.2. New York: Plenum Press.

Rosidah, I. 2010. Mikroenkapsula Fraksi Aktif Dari Herba Sambiloto (Adrographis paniculata Ness.) yang Berkhasiat Sitotoksik Dengan Metode Semprot Kering.(tesis).Depok. FMIPAUI.

Rugh, R. 1968. The Mouse its Reproduction and Development. Burgess Publishing Company: United State of America.

Sabri, E., D. Supriharti, dan G. E. Utama,. 2006. “Efek Pemberian Monosodium Glutamat (MSG) Terhadap Perkembangan Embrio Mencit (Mus musculus L.) Strain DDW Selama periode Praimplantasi Sampai Organogenesis.”Jurnal Biologi Sumatera. Vol 1 (1 ):8-14.

Setyawati, I. dan D.A.Yulihastuti. 2011. “Penampilan Reproduksi dan Perkembangan Skeleton Fetus Mencit Setelah Pemberian Ekstrak Buah Nanas Muda”. Jurnal Veteriner. Vol 12 (3): 192-199.

Setyawati, I. 2009. “Morfologi Fetus Mencit (Mus musculus L.) Setelah Pemberian Ekstrak Daun Sambiloto (Andrographis paniculata Ness.)”. Jurnal Biologi. Vol 13 (2): 41-44.

.[15]Siburian, J., dan R.Marlinza. 2009. “Efek Pemberian Ekstrak Akar Pasak Bumi (Eurycoma Longifolia Jack) Pada Tahap Prakopulasi Terhadap Fertilitas Mencit (Mus Musculus L.) Betina”. Biospecies. Vol 2 (2): 24-30.

Sumarmin, R. 2016. Perkembangan Hewan. Jakarta: Kencana.

Syahrum, H. M. 1994. Reproduksi dan Embriologi :Dari Satu Sel Menjadi Organisme. Jakarta : Fakultas Kedokteran Universitas Indonesia.

Tolistiawaty, I., J. Widjaja., P. P. F. Sumolang., dkk. 2014. “Gambaran Kesehatan pada Mencit (Mus musculus) di Instalasi Hewan Coba”. Jurnal Vektor Penyakit.Vol 8(1): 27-32.

Widiyawati, Tri. 2007. “Aspek Farmakologi Sambiloto”. Majalah Kedokteran Nusantara. Vol 40 (3): 216-222.

Wilson, J.G. 1973. Buku Teks Kimia Farmasi dan Medisinal Organik. Penerjemah, Achmad Mustafa Fatah (1982). Semarang: IKIP Semarang Press.

Yatim, Wildan. 1984. Embriologi Untuk Mahasiswa Biologi dan Kedokteran Bandung: Tarsito.




DOI: http://dx.doi.org/10.52155/ijpsat.v10.1.534

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Wendikal Abadi, Ramadhan Sumarmin

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.