Systematic Literature Review: The Aplication of Thin Cell Layer (TCL) Technique in Orchid Plant Propagation

Naura Muthiah Arli, Zozy Aneloi Noli

Abstract


Thin Cell Layer (TCL) is a technique using small thin sections produced from organ pieces. TCL explants more effective than large conventional explants. Thin explants facilitate contact and diffusion of media into the tissue, better than thick explant slices. This literature review aims to gather information related to the application of the thin cell layer technique in orchid plant propagation.  A systematic Literature Review (SLR) uses in this paper. TCL explants proved to be more effective than large conventional explants. Thin explants facilitate contact and diffusion of media into the tissue, better than thick explant slices. The TCL technique has been successfully used in the propagation of several orchid species including Paphiopedilum callosum, Cattleya forbesii, Dendrobium aduncum, Phalaenopsis hybrid, Brasilidium forbesii, Dendrobium aqueum, and Hadrolaelia grandis. The TCL technique both transversely and longitudinally has its own specificity with each type of explant used. In the TCL technique, protocorm is the most potential explant in orchid propagation. The development of explants using the TCL technique requires media supplemented with growth regulators. In general, the growth regulators commonly used in orchid propagation using the TCL technique are auxins and cytokinins.

Keywords


Orchid; propagation; thin cell layer

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References


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DOI: http://dx.doi.org/10.52155/ijpsat.v36.2.4928

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