TRAF6 and LC3 gene expressions in Colorectal cancer patients and Predicting metastasis using deep machine learning
Abstract
Method: To evaluate the gene expression level, thirty-two colorectal tumor samples and adjacent normal samples were used for carrying out the Real-Time PCR method. Also, a model based on deep CNN was designed for the prediction of metastatic symptoms in CRC patients.
Results: We reported that there was a significant difference in the expression level of TRAF6 and LC3 genes in tumor samples compared to normal samples (P<0.05). However, this study was designed as a pilot study, and further investigations are required to confirm our findings.
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Astin, M., Griffin, T., Neal, R. D., Rose, P., & Hamilton, W. (2011). The diagnostic value of symptoms for colorectal cancer in primary care: a systematic review. Br J Gen Pract, 61(586), e231-e243.
Burada, F., Nicoli, E. R., Ciurea, M. E., Uscatu, D. C., Ioana, M., & Gheonea, D. I. (2015). Autophagy in colorectal cancer: An important switch from physiology to pathology. World journal of gastrointestinal oncology, 7(11), 271.
Chung, J. Y., Park, Y. C., Ye, H., & Wu, H. (2002). All TRAFs are not created equal: common and distinct molecular mechanisms of TRAF-mediated signal transduction. Journal of cell science, 115(4), 679-688.
De Rosa, M., Pace, U., Rega, D., Costabile, V., Duraturo, F., Izzo, P., & Delrio, P. (2015). Genetics, diagnosis and management of colorectal cancer. Oncology reports, 34(3), 1087-1096.
Jiang, Z.-F., Shao, L.-J., Wang, W.-M., Yan, X.-B., & Liu, R.-Y. (2012). Decreased expression of Beclin-1 and LC3 in human lung cancer. Molecular biology reports, 39(1), 259-267.
Kondo, Y., Kanzawa, T., Sawaya, R., & Kondo, S. (2005). The role of autophagy in cancer development and response to therapy. Nature Reviews Cancer, 5(9), 726.
Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., . . . Yoshimori, T. (2000). LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. The EMBO journal, 19(21), 5720-5728.
Lee, N.-K., & Lee, S.-Y. (2002). Modulation of life and death by the tumor necrosis factor receptor-associated factors (TRAFs). BMB Reports, 35(1), 61-66.
Haggar, F. A., & Boushey, R. P. (2009). Colorectal cancer epidemiology: incidence, mortality, survival, and risk factors. Clinics in colon and rectal surgery, 22(4), 191.
Meng q, zheng m, liu h, song c, zhang w, yan j, qin l and liu x. 2012. TRAF6 regulates proliferation, apoptosis, and invasion of osteosarcoma cell. Molecular and cellular biochemistry 371: 177-186.
Rajandram, R., Bennett, N., Morais, C., Johnson, D., & Gobe, G. (2012). Renal cell carcinoma: resistance to therapy, role of apoptosis, and the prognostic and therapeutic target potential of TRAF proteins. Medical hypotheses, 78(2), 330-336.
Rong y, wang d, wu w, jin d, kuang t, ni x, zhang l and lou w. 2014. TRAF6 is over-expressed in pancreatic cancer and promotes the tumorigenicity of pancreatic cancer cells. Medical Oncology 31: 260.
Siegel, R. L., Miller, K. D., Fedewa, S. A., Ahnen, D. J., Meester, R. G., Barzi, A., & Jemal, A. (2017). Colorectal cancer statistics, 2017. CA: a cancer journal for clinicians, 67(3), 177-193.
Sun h, li x, fan l, wu g, li m and fang j. 2014. TRAF6 is upregulated in colon cancer and promotes proliferation of colon cancer cells. The international journal of biochemistry & cell biology 53: 195-201.
Shen, Y., Li, D.-D., Wang, L.-L., Deng, R., & Zhu, X.-F. (2008). Decreased expression of autophagy-related proteins in malignant epithelial ovarian cancer. Autophagy, 4(8), 1067-1068.
Tanida, I., Ueno, T., & Kominami, E. (2004). LC3 conjugation system in mammalian autophagy. The international journal of biochemistry & cell biology, 36(12), 2503-2518.
Zapata, J. M., Krajewska, M., Krajewski, S., Kitada, S., Welsh, K., Monks, A., . . . Gascoyne, R. D. (2000). TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies. The Journal of Immunology, 165(9), 5084-5096.
Yoshioka, A., Miyata, H., Doki, Y., Yamasaki, M., Sohma, I., Gotoh, K., ... & Monden, M. (2008). LC3, an autophagosome marker, is highly expressed in gastrointestinal cancers. International journal of oncology, 33(3), 461-468.
DOI: http://dx.doi.org/10.52155/ijpsat.v32.2.4307
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