The Application of Plant Growth Regulators in Propagation of Dendrobium Orchid with Thin Cell Layer (TCL) Technique: A Review

Naura Muthiah Arli, Zozy Aneloi Noli, M. Idris

Abstract


Plant growth regulators is one of the success factors for in vitro propagation. Plant growth regulators are organic compounds that are not nutrients and, in small amounts, promote, inhibit, or regulate physiological processes in plants. The function of growth regulators is to stimulate the growth of morphogenesis in cell, tissue and organ cultures. The most used plant growth regulators in tissue culture are auxins, cytokinins and gibberellins. One of technique for tissue culture is the thin cell layer technique. Thin Cell Layer (TCL) is a technique using small thin sections produced from organ pieces. TCL explants more effective than large conventional explants. Thin explants facilitate contact and diffusion of media into the tissue, better than thick explant slices. This literature review aims to gather information related to the application of plant growth regulators in propagation of Dendrobium orchid with thin cell layer (tcl) technique. A systematic Literature Review (SLR) uses in this paper. The growth regulators commonly used in Dendrobium orchid propagation using the TCL technique are auxins and cytokinins and the type of media that is often used in Dendrobium orchid propagation is Murashige and Skoog (MS). Protocorm Like Bodies (PLB) is the most potential explant in Dendrobium orchid propagation. The TCL technique both transverse and longitudinal has its own specificity with each type of explant used. TCL explants proved to be more effective than large conventional explants.

Keywords


Dendrobium; plant growth regulators; propagation; thin cell layer

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References


Nurcahyani, E., M.L. Lande., & R.A. Noviantia. “Induced Resistance of Moon Orchid Planlet (Phalaenopsis amabilis (L,) as Result of The In Vitro Salicylic Acid Selection Toward to Fusarium oxysporum”. Jurnal Penelitian Pertanian Terapan, vol. 17(2), pp. 132-137, 2017.

Agustini, V., I. Rahayu, L.A. Numberi, dan Z. Ni’mah. “Peran Chitosan Sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara In Vitro”. Jurnal Biologi Papua, vol. 12(1), pp. 43-49, 2020.

Isda, M. N & S. Fatonah. “Induksi Akar pada Eksplan Tunas Anggrek Grammatophylum scriptum var. citrinum secara In Vitro pada Media MS dengan Penambahan NAA Dan BAP”. Al-Kauniyah Jurnal Biologi, vol. 7(2), pp. 53-57, 2014.

Rahayu, T. “Modul Praktek Kultur Jaringan Tanaman”. Universitas Muhammadiyah Surakarta. Surakarta, 2016.

Deli, R.N., Z.A. Noli., & Suwirmen. “Respon Pertumbuhan Nodus Artemesia vulgaris L. Pada Medium Murashige-Skoog dengan Penambahan Beberapa Zat Pengatur Tumbuh Secara In Vitro”. Jurnal Biologi Universitas Andalas (J. Bio. UA), vol. 4(3), pp. 162-168, 2015.

Teixeira-da-Silva, J.A & Dobranszki, J. “Plant thin cell layers: A 40-year celebration”. Journal Plant Growth Regulator, vol. 43(32), pp. 922, 2013.

Agisimanto, D. “Thin Cell Layer mempercepat pembuatan populasi genotip unggul hortikultura”. Iptek Hortikultura, vol. 11, pp. 67-72, 2015.

Thanh, T.N., L.V. Diep., N.M. Trung., & T.T.B. Phoung. “Application of Thin Cell Layer Culture Method in Micropropagation on Dendrobium aduncum”. Journal of Biotechnology, vol. 8, pp. 361-367, 2010.

Parthibhan, S., M.V. Rao., J.A. Teixeira Da Silva, & T.S. Kumar. “Somatic Embryogenesis from Stem Thin Cell Layers of Dendrobium aqueum”. Biologia Plantarum, vol. 62(3), pp. 439-450, 2018.

Bhattacharyya, P., P. Paul., S. Kumaria., & P. Tandon. “Transverse thin cell layer (tTCL) mediated improvised micropropagation protocol for endangered medicinal orchid Dendrobium aphyllum Roxb: an integrated phytomoleculer approach”. Acta Physiologiae Plantarum, vol. 40(137), 2018.

Malik, A.N.B.A. “Proliferation and Elicitation of Protocorm Like Bodies od Dendrobium Sabin Blue for In Vitro Production of Anthocyanin”. Thesis. USM Malaysia, 2019. Serliana, Mukarlina, & R. Linda. “Pertumbuhan Anggrek Hitam (Coelogyne pandurata Lindl.) secara in vitro dengan Penambahan Ekstrak Tomat dan benzylaminopurine (BAP)”. Probiont, vol. 6(3), pp. 310-315, 2017.

Jaiphet, C & N. Rangsayatorn. “Micropropagation of a Rare Orchid Dendrobium gratiosissimum Using Thin Cell Layers”. ISHS Acta Horticulture, vol. 878(21), pp. 185-189, 2010.

Jaiphet, C. “In Vitro Conservation and Micropropagation of Dendrobium spp. and Eulophia spectabilis”. Thesis. University of Phayao Thailand, 2012.

Rangsayatorn, N. “Micropropagation of Dendrobium draconis Rchb. f. from Thin Cross-Section Culture”. Sci Hortic, vol. 122, pp. 662-665, 2009.

Zhao, P., W. Wang., F.S. Feng., F. Wu., Z.Q. Yang., & W.J. Wang. “ High Frequency Shoot Regeneration through Transverse Thin Cell Layer Culture in Dendrobium candidum Wall ex Lindl”. Plant Cell Tissue Organ Cult, vol. 90, pp. 131-139, 2007.

Nhat, N.T., & T.T. Dung. “In Vitro Propagation of Dendrobium orchid through Thin Stem Section Culture”. In: Proceedings of International Workshop on Biotechnology in Agriculture, Nong Lam University, Ho Chi Minh City, October 20-21, 2006.

Malabadi, R.B., G.S. Mulgund., & N. Kallappa. “Micropropagation of Dendrobium nobile from Shoot Tip Sections”. J. Plant Physiology, vol. 162, pp. 473-478, 2005.

Triandini, E., S. Jayanatha., A. Indrawan., G.W. Putra., & B. Iswara. “Metode Systematic Literature Review untuk Identifikasi Platform dan Metode Pengembangan Sistem Informasi di Indonesia”. Indonesian Journal of Information Systems, vol. 1(2), pp. 63-77, 2019.

Serliana, Mukarlina, & R. Linda. “Pertumbuhan Anggrek Hitam (Coelogyne pandurata Lindl.) secara in vitro dengan Penambahan Ekstrak Tomat dan benzylaminopurine (BAP)”. Probiont, vol. 6(3), pp. 310-315, 2017.

Nikmah, Z.C., W. Slamet, & B.A. Kristanto. “Aplikasi Silika dan NAA terhadap Pertumbuhan Anggrek Bulan (Phalaenopsis amabilis L.) pada Tahap Aklimatisasi”. J. Agro Complex, vol. 1(3), pp. 101-110, 2017.

Tuhuteru, S., M.L. Hehanussa, & S.H.T. Raharjo. “Pertumbuhan dan Perkembangan Anggrek Dendrobium anosmum pada Media Kultur In Vitro dengan Beberapa Konsentrasi Air Kelapa”. Agrologia, vol. 1(1), pp. 1-12, 2012.

Jualang, A.G., D. Devina, M. Hartinie, J.S. Sharon & J. Roslina. “Asymbiotic seeds germination and seedlings development of Vanda dearei”. Malaysian Applied Biology, vol. 43(2), pp. 25-33, 2014.

Kartikaningrum, S., P. Dewi, D. Minangsari, S. Rudy, & M.P. Yufdy. “Konservasi Anggrek Spesies Alam Menggunakan Eksplan Biji pada Media Vacin & Went”. Plasma Nutfah, vol. 23(2), pp. 109-118, 2017.

Fithriyandini, A., M.D. Maghfoer, & T. Wardiyati. “Pengaruh 6-Benzylaminopurine (BAP) terhadap Pertumbuhan dan Perkembangan Nodus Tangkai Bunga Anggrek Bulan (Phalaenopsis amabilis) dalam Perbanyakan secara In Vitro”. Produksi Tanaman, vol. 3, pp. 39-43, 2015.

Steinmacher, D.A., N.G. Krohn, A.C.M. Danras, V.M. Stefenon, C.R. Clement, & M.P. Guerra. “Somatic embryogenesis in peach palm using the thin cell layer technique: induction, morpho-histological aspects and AFLP analysis of somaclonal variation”. Annals of Botany, vol. (9)100, pp. 699, 2012.




DOI: http://dx.doi.org/10.52155/ijpsat.v39.2.5505

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