Micropropagation of Bulbophyllum Orchids

Syifa Ulia, Zozy Aneloi Noli, M. Idris

Abstract


Abstract— Bulbophyllum (Orchidaceae) is recognized as the largest genus of orchids in the world. Bulbophyllum is a popular orchid by its unique flowers and its benefits. Although Bulbophyllum is a large genus, research on in vitro propagation of orchids from this genus is still limited. This article aims to examine the micropropagation of several Bulbophyllum species to determine the affecting factor for the propagation of Bulbophyllum orchids. A systematic Literature Review (SLR) uses in this paper. The micropropagation of Bulbophyllum have studied in B.auricomum, B. fascinator, B. macrantum, B. phalaenopsis, B. lasianthum B. odoratissimum B. niphondii, B. dhaninivatii, B. lilacinum, B. echinolabium, B. putidum and B. plumatum. The use of appropriate media can increase the growth of orchids. The addition of growth regulators and/or organic compounds to a suitable basal medium can be a way to find a suitable medium for orchid growth. Acclimatization should be conducted on plantlets with healthy roots. The appropriate acclimatization media can increase the survival rate of orchid seedlings.


Keywords


Bulbophyllum; Micropropagation; Germination; Multiplication; Acclimatization.

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References


Comber, J.B, “Orchids of Sumatra”. The Royal Botanic Garden: Kew, 2001.

Vermeulen, J.J., P.J. O’Byrne & A. Lamb, “Bulbophyllum of Borneo. natural history publications (Borneo), Kota Kinabalu," Natural History Publication Borneo. Malaysia. 2015.

Metusala, D., “Bulbophyllum acehense (Orchidaceae), a new species of section Beccariana from Aceh, Sumatra, Indonesia,” Jurnal Biologi Tropis, vol. 20 (1), pp. 111 – 115. 2020.

Xinqi, C & J.J. Vermeulen, “Bulbophyllum Thouars,” Flora of China Vol 25, pp. 404-440. 2009.

Mitra, S & S.K.R. Mukherjee, “Diversity Of Genus Bulbophyllum Thouars In West Bengal - A Census,” J. Econ. Taxon. Bot. vol. 34 (1), pp. 224-234. 2010.

Than, M., A. Pal & S. Jha, “In Vitro Flowering and Propagation of Bulbophyllum auricomum Lindl., the Royal Flower of Myanmar”. Acta Horticulturae, vol. 829, pp. 105-111. 2009.

Lee Y.I. & E.C. “Yeung, Embryo development and in vitro seed germination of Bulbophyllum fascinator,” In: Blancard MG et al. (eds) Proceedings of Acta Hortivulturae 878 on I International Orchid Symposium in Taichung, Chinese Taipei, 12-15 January 2010.

Handayani, R., E. Handini, and P. Aprilianti, “The Effect of Plant Growth Regulator NAA for In Vitro Growth Optimization of Bulbophyllum spp,” Tropical Bioscience, vol. 2 (2), pp. 51-62. 2022.

Prasad, G., T. Seal, A.A. Mao, D. Vijayan and A. Lokho, “Assessment of clonal fidelity and phytomedicinal potential in micropropagated plants of Bulbophyllum odoratissimum - an endangered medicinal orchid of indo burma megabiodiversity hotspot,” South African Journal of Botany, vol. 141, pp. 487–497. 2021.

Pakum, W., S. Watthana, K. O. Srimuang, K and A. Kongbangkerd, “Influence of medium component on in vitro propagation of thai’s endangered orchid: Bulbophyllum nipondhii Seidenf.,” Plant Tissue Culture and Biotechnology, vol. 26 (1), pp. 37–46. 2016.

Kongbangkerd, A., S. Watthana, and K. Srimuang, “Influence of organic supplements on growth and development of in vitro shoot of Bulbophyllum dhaninivati Seidenf.,” Applied Mechanics and Material, vol. 855, pp. 42-46. 2017.

Aung, W.T., K.S. Bang, S.A. Yoon, B. Ko, & J.H. Bae, “Effect of different natural extracts and plant growth regulators on plant regeneration and callus induction from pseudobulbs explant through in vitro seed germination of endangered orchid Bulbophyllum auricomum Lindl.,” Journal of Bio-Environtment Control vol. 31 (2), pp. 133-141. 2022.

CITES, Appendices I, II and III. http://www.cites.org/eng/app/appendices. pdf. 20 januari 2022.

Budiharta, S., D. Widyatmoko, Irawati, H. W. Rugayah, T. Partomihardjo, Ismail, T. Uj i, A. P. Keim and K. A. Wilson, “The processes that threaten Indonesian Plants. fauna & flora international,” Oryx, vol. 45(2), pp. 172–179. 2011.

Lal, N. and Singh, M., “Prospect of plant tissue culture in orchid propagation: a. review” Indian Journal of Biology vol. 7 (2), pp. 103-110. 2020.

Eriksson, O., and K. Kainulainen, “The evolutionary ecology of dust seeds,” Perspectives in Plant Ecology, Evolution, and Systematics, vol. 13, pp. 73–87. 2011.

Jolman, D., M. I. Batalla, A. Hungerford, P. Norwood, N. Tait and L. E. Wallace, “The challenges of growing orchids from seed for conservation: an assessment of techniques. Application in Plant Science, vol. 10 (5), pp. 1-18. 2022.

Arditti, J. & A. Ghani. “Physical properties of orchid seeds and their biological implications,” New Phytologist vol. 145, pp. 367–421. 2000.

Rasmussen, H. N., “Terrestrial orchids from seed to mycotrophic plant,” United Kingdom: Cambridge University Press, Cambridge. 1995.

Lee, Y.I., C.F. Lu, M.C. Chung, E.C. Yeung, and N. Lee, “Developmental changes in endogenous abscisic acid concentrations and asymbiotic seed germination of a terrestrial orchid, Calanthe tricarinata Lindl.,” Journal of the American Society for Horticultural Science, vol. 132, pp. 246–252. 2007

Yeung, E. C. and D. W. Meinke, “embryogenesis in angiosperms: development of the suspensor,” The Plant Cell, vol. 5, pp. 1371-1381. 1993.

Freestone, M., C. Linde, N. Swarts and N. Reiter, “Asymbiotic germination of Prasophyllum (Orchidaceae) requires low mineral concentration,” Australian Journal of Botany, vol. 71 (2), pp. 67-78. 2023.

Sudheer, W.N., N. Praveen, J.M. Al-Khayri & S.M. Jain, “Role of plant tissue culture medium components, advances in plant tissue culture (current developments and future trends)”. India: Academic Press. 2022.

Bhatia, S., “Plant tissue culture, modern application of plant biotechnology in pharmaceutical science,” New York: Elsevier Inc. 2015.

Figura, T., M. Weiser & J. Ponert, “Orchid seed sensitivity to nitrate reflects habitat preferences and soil nitrate content,” Plant Biology, vol. 22(1), pp. 21–29. 2020.

Aprilianti, P., E. Handini. & D. M. Puspitaningtyas, “A seed morphometry study of selected species of Bulbophyllum and Dendrobium (Orchidaceae) in relation to their dispersals,” Biodiversitas, vol. 22 (12), pp. 5564-5571. 2021.

Hunhoff, V. L., L. A. Lage, E. G. Palu, W. Krause and C. E. Silva, “Nutritional requirements for germination and in vitro development of three orchidaceae spesies in the Southern Brazilian Amazon,” Ornamenta Horticulture, vol. 24 (2), pp. 87-94. 2018.

Hsu, R. C.C. and Y.L. Le, “Seed Development of Cypripedium debile Rchb. F. In relation to asymbiotic germination,” Horticultural Science, vol. 47 (10), pp. 1495-1498. 2012.

Caleco, J., A. Copetta, I. Marchioni, M. Bazzicalupo, M. Pianta, N. Shirmohammadi, L. Cornara and A. Giovannini, “The use of a new culture medium and organic supplement to improve in vitro early stage development of five orchid species,” Plant Biosystems, vol. 156, pp. 143-151. 2020.

Gaspar, T., C. Keveks, C. Penel, H. Greppin, D.M. Reid, T.A. Thorpe, “Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cellular and Development Biologi – Plant, vol. 32 (4), pp. 272–289. 1996.

Paramanik, P., D. K. Kar and S. Raha, “A review on asymbiotic seed germination in orchid through plant tissue culture,” Journal of Scientific Enquiry, vol. 1, pp. 1-7. 2021.

Masclaux-Daubresse C., F. Daniel-Vedele, J. Dechorgnat, F. Chardon, L. Gaufichon and A. Suzuki, “Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture,” Annals of Botany, vol. 105, pp. 1141–1157. 2010.

Swarts, N.D and K.W. Dixon, “Terrestrial orchid conservation in the age of extinction” Annals of Botany, vol. 104 (3), pp. 543–556 . 2009.

Swarts, N.D and K.W. Dixon, “Conservation methods for terrestrial orchids,” Ross Publishing, Richmond, 2017.

Cardoso J.C, C.A. Zanello and J.T. Chen, “An overview of orchid protocorm-like bodies: mass propagation, biotechnology, molecular aspects, and breeding,” International Journal of Molecular Science, vol. 21(3). 985. 2020.

Seswita, D., I. Mariska and E.G. Lestari, “Mikropropagasi nilam penampakan khimera hasil radiasi pada kalus,” Prosiding Pertemuan Ilmiah Aplikasi Isotop dan Radiasi. Jakarta, 9-10 Januari 1996.

Dwiyani, R., A. Purwantoro, A. Indrianto and E. Semiarti, “Konservasi anggrek alam Indonesia Vanda tricolor lindl. varietas suavis melalui kultur embrio secara in vitro,” Jurnal Bumi Lestari vol. 12 (1), pp. 93-98. 2012.

S. Tuhuteru, M.L. Hehanussa and S.H.T. Raharjo, “Pertumbuhan dan perkembangan anggrek Dendrobium anosmum pada media kultur in vitro dengan beberapa konsentrasi air kelapa,” Agrologia, vol. 1 (1), pp. 1-12. 2012.

Shekarriz, P., M. Kafi, S.D. Deilamy and M. Mirmasoumi, “Coconut water and peptone improve seed germination and protocorm like body formation of hybrid Phalaenopsis,” Agriculture Science Developments, vol. 2 (10), pp. 317-322. 2014.

Gupta, A., “Asymbiotic seed germination in orchids: role of organic additives,” International Advanced Research Journal in Science, Engineering and Technology, vol. 3, pp. 143-147. 2016.

Ambarwati, I.D., F.N. Alfian and P. Dewanti, “Dendrobium sp., Oncidium sp., and Phalaenopsis sp. Orchids response to addition of four different types of Organic Nutrients at Planlet Regeneration Stage,” Jurnal Agricultura, vol. 32 (1), pp. 27-36. 2021.

Amalia, R.T, Nurhidayati, and S. Nurfadilah, “Pengaruh jenis dan konsentrasi vitamin terhadap pertumbuhan dan perkembangan biji Dendrobium laxiflorum J. J Smith. secara in vitro,” Sains dan Seni Pomits, vol. 1, pp. 1-6. 2013.

Diaz, L.P., J.J. Namur, S.A. Bollati, O.E.A. Arce, “Acclimatization of Phalaenopsis and Cattleya obtained by micropropagation,” Revista Colombiana de Biotechnologia, vol. 12 (2), pp. 27–40. 2010

Kang, H., Kang, K.W., Kim, D.H., Sivanesan, I., “In vitro propagation of Gastrochilus matsuran (Makino) Schltr., an endangered epiphytic orchid,” Plants vol. 9 (4). 524-. 2020.

Utami, E.S.W., S. Hariyanto and Y.S.W. Manuhara, “In vitro propagation of the endangered medicinal orchid Dendrobium lasianthera J.J.Sm. through mature seed culture,” Asian Pasific Journal of Tropical Biomedicine, vol. 7(5), pp. 406-410. 2017.

Abbaszadeh, S. M., S. M. Miri and R. Naderi, “An effective nutrient medium for asymbiotic seed germination and in vitro seedling development of Phalaenopsis ‘Bahia Blanca’,” Journal of Ornamental Plants, vol. 8 (3), pp. 183-192. 2018.

Krisdianto, A., Saptiningsih, E., Nurchayati, Y. and N. Setiari, “Growth of Phalaenopsis ambilis (L.) Blume orchid plantlet on subculture stage by different of media types and pepton concentrations,” Journal of Biologi Science, vol. 7 (2), pp. 182-190. 2020.

Umbeck, P.F. & K. Norstog, “Effects of Abscisic Acid and Ammonium Ion on Morphogenesis of Cultured Barley Embryos. Bulletin of Torrey Botanical Club, vol. 106 (2), pp. 110–116. 1979.

Yeung, E.C., “A perspective on orchid seed and protocorm development. Botanical Studies, vol. 58 (1), pp. 1–14. 2017.

Setiaji, A., N. Setiari, E. Semiarti, “Induksi tunas dari protokorm intak dan fase awal perkembangan Dendrobium phalaenopsis secara in vitro,” In: Setyawan, A. D., Sugiarto, A. Pitoyo, Sutomo, A. Widiastuti, G. Windarsih, Supatmi. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. Universitas Sebelas Maret. 18 September 2021. Masyarakat Biodiversitas Indonesia, vol. 4(1), pp. 20-27. 2018.

Guo, Y., G. Ren, K. Zhang, Z. Li, Y. Miao and H. Guo, “Lead senescence: progression, regulation, and application,” Molecular Horticulture, vol. 1 (5), pp. 1-25. 2021.

Puspitaningtyas, D. M. & E. Handini, “Seed germination evaluation of Phalaenopsis amabilis in various media for long-term conservation,” Biodiversitas vol. 22 (11), pp. 5231-5238. 2021.

Lokstein, H., G. Renger & J. P. Götze, “Photosynthetic light-harvesting (antenna) complexes-structures and functions,” Molecules vol. 26 (11), pp. 1-24. 2021.

Ulia, S, “ Pengaruh media dan sistem kultur dalam propagasi anggrek spesies Sumatra, Bulbophyllum putidum J.J.Sm. melalui teknik in vitro,” unpublished.

Warseno, T., E. Hendriyani, and A. Priyadi, “ Conservation and Propagation of Bulbophyllum echinolabium J.J.Sm. through in vitro culture,” Conference: Prosiding Ekpose Pembangunan Kebun Raya dan Seminar Konservasi Flora Indonesia. pp. 905 -916. 2013.

Bhadra, S.K., H. Barua and M.M. Hossain, “In vitro germination and rapid micropropagation of Bulbophyllum lilacinum Ridley.,” Bangladesh Journal Botani, vol. 33 (2), pp. 103-107. 2004.




DOI: http://dx.doi.org/10.52155/ijpsat.v39.2.5492

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