Optimization Of Antibiotics Production In Bacillus Subtilis Soil Strain G2B Against Staphylococcus Aureus And Escherichia Coli

Sindy Gemaeka Putri, Periadnadi Periadnadi, Yusra Yusra

Abstract


Bacillus subtilis strain G2B is a potential bacterium derived from anchovy (Stolephorus sp.) processing wastewater. This study aims to analyze the antimicrobial activity of Bacillus subtilis strain G2B in the production of antibiotics against Staphylococcus aureus and Escherichia coli test bacteria. The method used in this research is a survey method, the data is analyzed descriptively quantitatively. The results showed that Bacillus subtilis strain G2B had the ability to produce antibiotics as measured by its antibiosis index, which was 12 mm against Staphylococcus aureus bacteria and 13 mm against Escherichia coli test bacteria. The inhibition zone of Bacillus subtilis strain G2B against the test bacteria belongs to the strong category.

Keywords


Production, Antibiotics, Bacillus subtilis strain G2B, Waste

Full Text:

PDF

References


Axelsson, L., 2004. Lactic Acid Bacteria: Classification and Physiology. In: Salminen, S. von Wright, A. and Ouwehand A. (Eds.), lactic acid bacteria: microbiological and functional aspects. 3rd rev. and exp. ed. Marcel Dekker, Inc., New York, pp. 1-66.

Cappuccino, J. G and N. Sherman 2014. Microbiology a Laboratory Manual 10th Ed. San Francisco : Pearson Education, Inc. Publishing as Benjamin Cummings.

Defnur. A. 2019. Isolation, Potential and Characterization of Antibiosis Bacteria From Cattle Slaughterhouse Wastewater Against Staphylococcus aureus Rosenbach Bacteria. (Thesis) Faculty of Mathematics and Natural Sciences, Andalas University.

Diaz, J. R., 1993. Plantaricin S And T, Two New Bacteriocin Produced By Lactobacillus Plantarum LPC010 Isolated From A Green Olive Fermentation. Journal of Applied Environmental of Microbiology, 59:1416-1429.

Fardiaz, S. 1989. Food Microbiology. Bogor: Inter-University Center.

Ha, Y. M., Y. H. Park and Y. J. Kim., 2002. A taxonomic study of Bacillus sp. isolated from Korean salt fermented Anchovies. Molecular Biology Today, 3(1): 25-29.

Handayani. 2020. Isolation of Antibiotic-Producing Microbes from Lemo-Lemo Beach Sand, Bulukumba Regency in Inhibiting Several Pathogenic Bacteria. Journal of Health, 13(1):21-27.

Hatmanti, A. 2000. Introduction of Bacillus spp. Jakarta: Research and Development for Marine Environment LIPI.

Hutabarat, S. and Evans. 2012. Introduction to Oceanography. Jakarta : UI-Press.

Lay, B. W. 1994. Microbial Analysis in the Laboratory. Jakarta: Raja Grafindo Persada.

Nofisulastri, Z., Bachruddin and E. Harmayanti, 2006. Production and extraction of antibacterial bacteriocin from Pediococcus sp. NWD 015. Indonesian Journal of Biotechnology, 11(2): 921-927.

Nur, F. 2019. Isolation and Characterization of Halophilic Microbes Producing Extracellular Proteases from Bledug Kuwu Mud, Central Java. (Thesis). Department of Chemistry FMIPA, State University of Malang

Nursyirwani, Effendi. I and Desi, M. S. 2019. Molecular Identification of Antibiotic-Producing Bacteria from Extreme Microhabitats in Mangrove Ecosystems and Their Activities Against Pathogenic Bacteria (Vibrio alginolyticus). Journal of Fisheries and Maritime Affairs, 9(2): 137-150.

Periadnadi, Nurmiati and H. A. Defnur. 2021. Optimization of Antibiotic Production On Various Protein Substrates Against Staphylococcus Aureus And Escherichia coli. International Journal of Progressive Science and Technologies (IJPSAT). 28(2): 169-173.

Rifai. M, H. Widowati and A. Sutanto. 2020. Synergism and Antagonism of Several Types of Consumed Bacterial Isolates. Journal of Biolova 1(1). 21-26.

Sabdaningsih. A., and Arina T. L. 2020. Isolation and Morphological Characterization of Halophilic Bacteria from Bledug Kuwu, Grobogan Regency. Journal of Biomes, 22(1): 46-52.

Setiaji, J. 2019. DNA Analysis of Marine Heterotrophic Bacteria That Are Antagonists Against Pathogenic Bacteria in Fish. Proceedings of the Seminacle, 1(1): 17-27.

Surdjowardjojo. P., Susilorini T. E., Sirait G. R. B. 2015. Inhibition of Decoction of Manalagi Apple Skin (Mulus cylvertrs Mill) on the Growth of Staphylococcus aureus and Pseudomonas sp. Journal of Tropical Livestock. 16(2): 40-48.

Torkar. K.G and B.B. Matijasic. 2003. Partial Characterization Of Bacteriocins Produced By Bacillus Cereus Isolates From Milk And Milk Products. Journal of Food Technology Biotechnology, 41(2): 121–129.

Yusra and Y. Efendi . 2010. Fundamentals of Fishery Product Technology. Padang: Bung Hatta University Press.

Yusra, Y. Efendi and A. Mardiah. 2019. Training on Fish Handling and Processing in Pasia Nan Tigo Village, Koto Tangah District, Padang City. Vocational Journal, 3(2):105-111.

Yusra., F. Azima,. Novelina and Periadnadi. 2014. Antimicrobial Activity Of Lactic Acid Bacteria Isolated From Budu Of West Sumatra To Food Biopreservatives. Pakistan Journal of Nutrition, 12(7): 628- 635.

Zafrana, S. Ismia , I. Mastutia and K. Mahardika. 2021. Isolation and Characterization of Bacteria Isolated from Larvae of Beautiful Hybrid Grouper Fish Affected by Stunted Tail Disease. Journal of Fisheries and Marine Research, 4(2):194-205.




DOI: http://dx.doi.org/10.52155/ijpsat.v37.1.5097

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 sindy gemaeka putri, Periadnadi Periadnadi, Yusra Yusra

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.