Effect of Biopriming with Padina Minor Seaweed Extract with Amino Acid Addition on Germination of Several Varieties of Rice (Oryza Sativa L.)

Widia Putri Febriani, Zozy Aneloi Noli, Mansyurdin Mansyurdin


Seed germination is a crucial step in plant propagation, as it controls seedling production, stand establishment, and crop yield. The current study evaluated the effect of seaweed extract (Padina minor) with the addition of amino acids biopriming on the seed germination of three (Cinta, Banang Pulau, and upland rice UNSOED 1) rice varieties. This study used a Randomized Completely Factorial Design consisting of 2 factors and four replications. Factor A (Biostimulant) consisting of a0) distilled water, a1) P.minor extract, a2) P.minor extract + amino acid (Glycine 250 ppm, Alanine 20 ppm, Cysteine 50 ppm, Arginine 50 ppm). Factor B (Rice variety) consists of b1) upland rice Unsoed 1, b2) Cinta (Solok local varieties) , and b3) Banang Pulau (Solok local varieties). The germination responses of the bio-primed seeds were measured using six parameters, including the percentage of germination, vigor index, maximum growth potential, hypocotyl, and root length. This study showed that P.minor extract is effectively used as bio-priming for several rice varieties. Upland rice variety Inpago UNSOED 1 gave the best response to priming treatment compared to Solok rice varieties Cinta and Banang Pulau. 


seed priming, seaweed extract, Amino Acid, Germination, Rice.

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A. A. Ahmed, “Improving seed germination and seedling growth of maize ( Zea mays L .) seed by soaking in water and moringa oleifera leaf extract” Curr. Chem. Lett., vol. 11, pp. 147–156, 2022, doi: 10.5267/j.ccl.2022.2.005.

P. P. Reddy, “Recent advances in crop protection,” Recent Adv. Crop Prot., pp. 1–268, 2013, doi: 10.1007/978-81-322-0723-8.

A. J. Deshmukh, R. S. Jaiman, R. P. Bambharolia, and V. A. Patil, “Seed Biopriming– A Review,” Int. J. Econ. Plants, vol. 7, no. 1, pp. 038–043, 2020, doi: 10.23910/2/2020.0359.

Carvalho M.E.A., N. A. D. C. Castro P.R.C., and H. M. C. P. Chamma, “Seaweed Extract Improves the Vigor and Provides the Rapid Emergence of Dry Bean Seeds,” J. Agric. Environ. Sci, vol. 13, no. 8, pp. 1104–1107, 2013, doi: 10.5829/idosi.aejaes.2013.13.08.11015.

G. Kumar and D. Sahoo, “Effect of seaweed liquid extract on growth and yield of Triticum aestivum var. Pusa Gold,” J. Appl. Phycol., vol. 23, no. 2, pp. 251–255, 2011, doi: 10.1007/s10811-011-9660-9.

K. R. Matysiak Kinga, Kaczmarek Sylwia, “Influence of seaweed extracts and mixture of humic and fulvic acids on germination and growth of Zea mays L.,” ACTA Sci. Pol., vol. 10, no. 1, pp. 33–45, 2011.

D. Mondal, A. Ghosh, K. Prasad, and S. Singh, “Elimination of gibberellin from Kappaphycus alvarezii seaweed sap foliar spray enhances corn stover production without compromising the grain yield advantage,” 2014, doi: 10.1007/s10725-014-9967-z.

W. A. Stirk, K. R. R. Rengasamy, M. G. Kulkarni, and J. van Staden, “Plant biostimulants from seaweed: An overview,” Chem. Biol. Plant Biostimulants, pp. 31–55, 2020.

M. El-Sheekh, M. Ismail, and M. Hamouda, “Influence of Some Brown Seaweed Extracts on Germination and Cytological Responses of Trigonella foenum-graecum L.,” Biotechnol. An Indian J. Res. |, vol. 12, no. 6, pp. 1–12, 2016, [Online]. Available: www.tsijournals.com

M. Eugenia et al., “Seaweed Extract Improves the Vigor and Provides the Rapid Emergence of Dry Bean Seeds,” J. Agric. Environ. Sci, vol. 13, no. 8, pp. 1104–1107, 2013, doi: 10.5281/zenodo.51608.

H. Maeda and N. Dudareva, “The shikimate pathway and aromatic amino acid biosynthesis in plants,” Annu. Rev. Plant Biol., vol. 63, pp. 73–105, 2012, doi: 10.1146/annurev-arplant-042811-105439.

W. F. Teixeira, E. B. Fagan, L. H. Soares, and R. C. Umburanas, “Foliar and Seed Application of Amino Acids Affects the Antioxidant Metabolism of the Soybean Crop,” Front. Plant Sci., vol. 8, no. March, pp. 1–14, 2017, doi: 10.3389/fpls.2017.00327.

H. A. A. Hussein, S. O. Alshammari, S. K. M. Kenawy, F. M. Elkady, and A. A. Badawy, “Grain-Priming with L-Arginine Improves the Growth Performance of Wheat (Triticum aestivum L.) Plants under Drought Stress,” Plants, vol. 11, no. 9, 2022, doi: 10.3390/plants11091219.

E. BARCANU et al., “The Effects of Seed Priming with L-glutamine and L-arginine Amino Acids in Pepper Seeds,” Bull. Univ. Agric. Sci. Vet. Med. Cluj-Napoca. Hortic., vol. 77, no. 2, p. 122, 2020, doi: 10.15835/buasvmcn-hort:2020.0040.

W. A. Stirk, D. Tarkowská, V. Turečová, M. Strnad, and J. van Staden, “Abscisic acid, gibberellins and brassinosteroids in Kelpak®, a commercial seaweed extract made from Ecklonia maxima,” J. Appl. Phycol., vol. 26, no. 1, pp. 561–567, 2014, doi: 10.1007/s10811-013-0062-z.

J. S. Mangmang, R. Deaker, and G. Rogers, “Early seedling growth response of lettuce , tomato and cucumber to Azospirillum brasilense inoculated by soaking and drenching,” Hort. Sci, vol. 42, pp. 37–46, 2015, doi: 10.17221/159/2014-HORTSCI.

R. Asra, “Pengaruh Hormon Giberelin (GA 3 ) Terhadap Daya Kecambah dan Vigoritas Calopogonium caeruleum Effect of Gibberellins (GA 3 ) Hormones to Germination and Vigority of Calopogonium caeruleum,” Biospecies, vol. 7, no. 1, pp. 29–33, 2014, [Online]. Available: https://online-journal.unja.ac.id/index.php/biospecies/article/view/1507

D. Weiss and N. Ori, “Mechanisms of cross talk between gibberellin and other hormones,” Plant Physiol., vol. 144, no. 3, pp. 1240–1246, 2007, doi: 10.1104/pp.107.100370.

W. A. Mary Shamya Arokia rajan, Rajasekar Thriunavukkarasu, Jerrine Joseph, “Effect of seaweed on seed germination and biochemical constituents of Capsicum annuum,” Biocatal. Agric. Biotechnol., vol. 29, p. 101761, 2020, doi: 10.1016/j.bcab.2020.101761.

D. Danial, E. Widajati, and S. Salma, “Efektivitas aplikasi,” Pros. Semin. Has. Penelit. Tanam. Aneka Kacang dan Umbi, pp. 73–81, 2013.

DOI: http://dx.doi.org/10.52155/ijpsat.v36.2.4950


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