Email this article 
Effect Of Thymus Vulgaris, Mentha Piperita And Pelargonium Citrosum Leaf, Syzygium Aromaticum Buds And Citrus Limonoids Peels Extracts As Mosquito Larvicidal And Pupicidal Agent
Abstract
Mosquito control depends on application of synthetic insecticides. This has been limited due to its harmful effect on human health. This increased needs for botanical origin alternatives as a sustainable and more safe method of mosquito control. The current study aimed to evaluate larvicidal and pupicidal effect of thyme (Thymus vulgaris), peppermint (Mentha piperita) and citronella leaf (Pelargonium citrosum), clove buds (Syzygium aromaticum) and lemon peels (Citrus limonoids) methanolic extracts. Tested plants were extracted and three concentrations of these extracts were prepared (25, 50 and 100 mg/ml) then their larvicidal and pupicidal activities were evaluated against 20 larvae and 20 pupas for each concentration. Mortality rate were calculated after 24 hrs. Adult mosquitoes were identified to genus level. Clove showed the highest larvae mortality rate (100%) for the three tested concentrations, followed by thyme, lemon and peppermint. Citronella had no effect as larvicidal agent. On the other hand, pupicidal activity test of thyme showed the highest results followed by clove. While lemon didn’t show any activity. Mosquito was identified as Culex. The result showed that buds extract of S. aromaticum and leaf extracts of T. vulgaris and peels extract of C. limonoids could serve as potential larvicidal extracts against the Culex mosquito.
Keywords
Full Text:
PDFReferences
Ghosh A., Chowdhury N. and Chandra G., 2012. Plant extracts as potential mosquito larvicides. Indi-an J Med Res. 135(5):581-598.
Brown AW.,1986. Insecticide resistance in mosquitoes: a pragmatic review. J Am Mosq Control As-soc. 2(2):123-40.
Russell TL., Kay BH. and Skilleter GA., 2009. Environmental effects of mosquito insecticides on saltmarsh invertebrate fauna. Aquat Biol. 6:77-90.
Roy A. and Saraf S. Limonoids: 2006. overview of significant bioactive triterpenes distributed in plants kingdom. Bio and Pharma Bulletin. 29(2):191–201.
Senthilkumar N., Varma P. and Gurusubramanian G., 2009. Larvicidal and adulticidal activities of some medicinal plants against the Malarial Vector, Anopheles stephensi (Liston). Parasitol Res. 104(2):237–244.
Dahchar Z., Bendali-Saoudi F. and Soltani N., 2016. Larvicidal activity of some plant extracts against two mosquito species Culex pipiens and Culiseta longiareolata. Journal of Entomology and Zoology Studies. 4(4):346-350.
Nakahara K., Alzoreky N. and Yoshihashi T., 2003. Chemical composition and antifungal activity of essential oil from Cymbopogon nardus (citronella grass). JARQ. 37(4):249–252.
Krishnappa K., Elumalai K., Dhanasekaran S. and Gokulakrishnan J., 2012. Larvicidal and repellent properties of Adansonia digitata against medically important human malarial vector mosquito Anopheles stephensi (Diptera: Culicidae). J. Vector Borne Dis. 49(2): 86–90.
Wahyuni D., 2012. Larvicidal Activity of Essential Oils of Piper betle from the Indonesian Plants against Aedes Aegypti L. J. Appl. Environ. Biol. Sci. 2(6):249–254.
Thomas A., Humphrey D., Manjurano A., Morona D. and Eliningaya J., 2017. Evaluation of active ingredients and larvicidal activity of clove and cinnamon essential oils against Anopheles gambiae (sensu lato). Parasites & Vectors. 10(1):411.
Kumar S., Wahab N. and Warikoo R., 2011. Bioefficacy of Mentha piperita essential oil against dengue fever mosquito Aedes aegypti L. Asian Pac J Trop Biomed. 1(2):85-88.
Abbott WS., 1987. A method of computing the effectiveness of an insecticide. J Am Mosq Control Assoc. 3(2):302-303.
Susheela PR. and Padmapriyanga S., 2016. Evaluation of larvicidal action of natural extracts on mosquito larvae of Aedes aegypti (Diptera: Culicidae). International Journal of Mosquito Research. 3(6):26-30.
Simas Nk., Lima ED., Conceição SD., Kuster RM., Oliveira AM. and Lage CLS., 2004. Natural products for dengue transmission control - Larvicidal activity of Myroxylon balsamum (red oil) and of terpencids and phenylpropanoids. Quim Nova. 27(1):46–49.
Massebo F., Tadesse M., Bekele T., Balkew M. and Gebre-michael T., 2009. Evaluation on larvi-cidal effects of essential oils of some local plants against Anopheles arabiensis Patton and Aedes aegypti Linnaeus (Diptera, Culicidae) in Ethiopia. African J. Biotechnol. 8(17):4183-4188.
Pavela R., 2008. Larvicidal effects of various Euro-Asiatic plants against Culex quinquefasciatus Say larvae (Diptera: Culicidae). Parasitol. Res. 102(3):555-559.
Dias CN. and Moraes DFC., 2014. Essential oils and their compounds as Aedes aegypti L. (Diptera: Culicidae) larvicides. review Parasitol. Res. 113(2):565-592.
Govindarajan M., Sivakumar R., Rajeswary M. and Veerakumar K ., 2013. Mosquito larvicidal ac-tivity of thymol from essential oil of Coleus aromaticus Benth. against Culex tritaeniorhynchus, Aedes albopictus, and Anopheles subpictus (Diptera: Culicidae). Send to Parasitol Res. 112(11):3713-21
Wahyuni D., 2012. Larvicidal Activity of Essential Oils of Piper betle from the Indonesian Plants against Aedes Aegypti L. J. Appl. Environ. Biol. Sci. 2(6):249–254.
Ansaria MA., Vasudev P., Tandonb M. and Razdana R., 2000. Larvicidal and mosquito repellent action of peppermint (Mentha piperita) oil. Bioresource Technology. 71(3):267-271.
Matsuda B., Surgeoner G., Heal J., Tucker A. and Maciarello M., 1996. Essential oil analysis and field evaluation of the citrosa plant "Pelargonium citrosum" as a repellent against populations of Aedes mosquitoes. J Am Mosq Control Assoc. 12(1):69-74.
Cilek J. and Schreiber E., 1994., Failure of the "mosquito plant", Pelargonium x citrosum 'van Leenii', to repel adult Aedes albopictus and Culex quinquefasciatus in Florida. J Am Mosq Control As-soc. 10(4):473-6.
DOI: http://dx.doi.org/10.52155/ijpsat.v28.2.3436
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Sakina Salem Saadawi
This work is licensed under a Creative Commons Attribution 4.0 International License.