Characterization and Differentiation of some Clinical Isolates from Urine

Magda Mohamed Awad, Amro Mohamed Mowafy, Yehia A. Osman

Abstract


E. coli is mostly a non-harmful bacterium which lives in the intestines of humans and animals. Some strains of this bacterium cause diseases to their hosts that require medical attention.  Children are the most vulnerable group and urine samples from those attended the outpatient clinics at Mansoura University, Egypt were used to isolate E. coli. The seventeen isolates were subjected to primary identification by Gram staining, growth on MacConkey agar media, and then followed by biochemical identification using catalase test, indole, methyl red, Vogas-Proskauer, and citrate tests.  Comparison of the protein profiles helped in the process of differentiation and characterization of the seventeen isolates. Some of the selected isolates were subjected to the three tests of coliform and E. coli test (Multiple-tube method (MPN), confirmed test and completed test). All the E. coli isolates were Gram-negative, short-rod, produced bright pink colonies on MacConkey agar, catalase positive, indole and methyl red positives, while, they were negatives to Vogas-Proskauer and citrate tests. After the protein profile, six isolates were selected for further studies. All six isolates indicated a positive MPN test, characteristic metallic sheen colonies on the Eosine Methylene Blue (EMB) agar within the confirmed test.


Keywords


E. coli, Enterobacteriaceae, urine, biochemical identification, and coliform test.

Full Text:

PDF

References


Abdallah, M. S., Mustapha, T., Gambo, A., and Ishaq, S. 2014. Biochemical identification and cultural characterization of some Gram-negative bacteria obtained from fecal/Diarrhoeal samples. CIBTech Journal of Microbiology ISSN: 2319-3867 (Online)

Ahmed, T., Baidya, S., Acharjee, M. and Rahman, T., 2013. Qualitative analysis of drinking water through the most probable number (MPN) method. Stamford Journal of Microbiology, 3(1), pp.9-16.

Alexander, M., 1983. Most probable number method for microbial populations. Methods of Soil Analysis: Part 2 Chemical and Microbiological Properties, 9, pp.815-820.

Barcella, L., Barbaro, A.P. and Rogolino, S.B., 2016. Colonial morphology of Escherichia coli: impact of detection in clinical specimens. Microbiologia

Medica, 31(2).

Bumadian, M.M., Almansury, H.H., Bozakouk, I.H., Lawgali, Y.F. and Bleiblo, F.A., 2013. Detection and Enumeration of Coliform Bacteria in Drinking Water at Hospital of Benghazi/Libya. J Exp Biol, 1(6), pp.437440.

Cheesbrough, M., 1985. Medical laboratory manual for tropical countries. Vol. II. Microbiology PP. 400-408.

Cheesbrough M. 2000. District Laboratory Practice in Tropical Countries Low Price edition, (UK, Cambridge shire, Britain Cambridge University Press) 62 -70.

Chelikani, P., Fita, I. and Loewen, P.C., 2004. Diversity of structures and properties among catalases. Cellular and Molecular Life Sciences CMLS, 61(2), pp.192-208.

Feng, P., Weagant, S.D., Grant, M.A., Burkhardt, W., Shellfish, M. and Water, B., 2002. BAM: Enumeration of Escherichia coli and the Coliform Bacteria. Bacteriological analytical manual, 13.

Forbes, B.A., Sahm, D.F. and Weissfeld, A.S., 2007. Bailey & Scott's Diagnostic Microbiology 12th Edition: Mosby

Elsevier. St. Louis. USA.

Kaper, J.B., Nataro, J.P. and Mobley, H.L., 2004. Pathogenic Escherichia coli. Nature reviews microbiology, 2(2), pp.123-140.

Kithar R. Majeed, Talib A. Jaayid and Salah N.A. Al-Khaeun. 2016. Isolation and Identification of some Types Bacteria from Shrimp (Metapenaeus affinis) and Detection of Histamine Producing from its Metapenaeus affinis. Basrah Journal of Agricultural Sciences. ISSN: 18175868, 29 (1) P.35-56.

Kravitz, J.D., Nyaphisi, M., Mandel, R. and Petersen, E., 1999. Quantitative bacterial examination of domestic water supplies in the Lesotho Highlands: water quality, sanitation, and village health. Bulletin of the World Health Organization, 77(10), p.829.

Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. nature, 227, 680-685.

Loewen, P.C., Switala, J. and Triggs-Raine, B.L., 1985. Catalases HPI and HPII in Escherichia coli are induced independently. Arch. Biochem. Biophys. 243(1), pp.144-149.

MacFadden, J.F. 2000. Biochemical tests for Identification of Medical Bacteria 3rd Ed. The Williams & Wilkins Co., USA: 689 – 691.

Collee, J. G., Mackie, T. J., and Mccartney, J. E. 1996. Mackie and McCartney practical medical microbiology. New York, Churchill Livingstone

Mohammed, Z.A. and Abd AL-MuhsinAL-

Khyat, F., 2008. Isolation and Identification of Escherichia coli O157: H7 from locally minced meat and imported minced and chicken meat. The Iraqi Journal of Veterinary Medicine, 32(1), pp.100-113.

Ryan, K.J., Ray, C.G., Ahmad, N., Drew, W.L. and Plorde, J.J., 2010. Chapter 33: Enterobacteriaceae. Sherris Medical Microbiology: An Introduction to Infectious Diseases, pp.579-608.

SAADI AL-BAER, A. & HUSSEIN, A. A. 2017. Isolation and Identification of Escherichia coli Producing Cytosine Deaminase from Iraqi patients. Int. J. Adv. Res. Biol. Sci, 4, 1-6.

Van Belkum, A., Welker, M., Erhard, M. and Chatellier, S., 2012. Biomedical mass spectrometry in today's and tomorrow's clinical microbiology laboratories. Journal of clinical microbiology, 50(5), pp.15131517.




DOI: http://dx.doi.org/10.52155/ijpsat.v23.1.2267

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Magda Mohamed Awad, Amro Mohamed Mowafy, Yehia A. Osman

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.