Random Differentiation of Bone Marrow Derived Mesenchymal Stem Cells

Mahmoud Elhussiny Salama, Mohamed Elshahat Ebaid, Tamer Mohamed Roshdy, Mohamed Sobh

Abstract


AIM: To investigate and determine the capacity of mesenchymal stem cells (MSCs) toward differentiation and to which extent they can differentiate.

METHODS: MSCs were isolated from the bone marrow of Sprague dewaly rats, and tested for their self-renewal ability and then characterized using flowcytometer. For differentiation, cells were seeded in low density for 3 monthos. The morphology observed daily and documented.

RESULTS: cells propagated in culture for more than 30 passages , MSCs showed a high capacity for differentiated into adipocyte, osteocyte and neural cells after different time.

 CONCLUSION: Bone marrow MSCs have great differentiation capabilities. MSCs can differentiate into functional cell lines, and may be used as a treatment for many diseases.


Keywords


stem cell, random differentiation, neural cells, Adipocyte and osteocyte.

Full Text:

PDF

References


REFERENCES

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284: 143-147.

Weissman IL. Stem cells: units of development, units of regeneration, and units in evolution Cell 2000; 100:157-168.

Tang DQ, Cao LZ, Burkhardt BR, Xia CQ, Litherland SA, Atkinson MA, et al. In vivo and in vitro characterization of insulin-producing cells obtained from murine bone marrow. Diabetes 2004; 53: 1721-1732.

Ianus A, Holz GG, Theise ND, Hussain MA. In vivo derivation of glucose competent pancreatic endocrine cells from bone marrow without evidence of cell fusion. J Clin Invest 2003; 111: 799-801.

Taneera J, Rosengren A, Renstrom E, Nygren JM, Serup P, Rorsman P, et al. Failure of transplanted bone marrow cells to adopt a pancreatic β-cells fate. Diabetes 2006; 55: 290-296.

Lechner A, Yang YG, Blacken RA, Wang L, Nolan AL, Habener JF. No evidence for significant transdifferentiation of bone marrow into pancreatic β-cells in vivo. Diabetes 2004; 53:616-623.

Haynesworth, S.E.; Baber, M.A.; Caplan, A.I. (1992) Bone, 13, 69.

Pittenger MF, Mackay AM, Beck SC et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143–147.

Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol 2000;28:875–884.

Timper K, Seboek D, Eberhardt M, Linscheid P, Christ-Crain M, Keller U, et al. Human adipose tissue-derived mesenchymal stem cells differentiate into insulin,

somatostatin, and glucagon expressing cells. Biochem Biophys Res Commun 2006; 341: 135-1140.

Zhang Y, Li CD, Jiang XX, Li HL, Tang PH, Mao N. Comparison of mesenchymal stem cells from human placenta and bone marrow. Chin Med J 2004; 117:882-887.

M.W. Lee, J. Choi, M.S. Yang, Y.J. Moon, J.S. Park, H.C. Kim, Y.J. Kim, Mesenchymal stem cells from cryopreserved human umbilical cord blood, Biochem. Biophys. Res. Commun. 320 (2004) 273–278.

Sanchez-Ramos J, Song S, Cardozo-Pelaez F et al. Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol 2000;164:247–256.

Woodbury D, Schwarz EJ, Prockop DJ et al. Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 2000;61:364–370.

Jiang Y, Jahagirdar BN, Reinhardt RL et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002;418:41–49.

Petersen, B. E., Bowen, W. C., Patrene, K. D., Mars, W. M., Sullivan, A. K., Murase, N., Boggs, S. S., Greenberger, J. S., and Goff, J. P. (1999) Bone marrow as a potential source of hepatic oval cells. Science 284, 1168–1170.

Theise, N. D., Badve, S., Saxena, R., Henegariu, O., Sell, S., Crawford, J. M., and Krause, D. S. (2000) Derivation of hepatocytes from bone marrow cells in mice after radiation-induced myeloablation. Hepatology 31, 235–240.

Theise, N. D., Nimmakayalu, M., Gardner R., Illei, P. B., Morgan, G., Teperman, L., Henegariu, O., and Krause, D. S. (2000) Liver from bone marrow in humans. Hepatology 32, 11–16.

Alison, M. R., Poulsom, R., Jeffery, R., Dhillon, A. P., Quaglia, A., Jacob, J., Novelli, M., Prentice, G., Williamson, J., and Wright, N. A. (2000) Hepatocytes from non-hepatic adult stem cells. Nature 406, 257.

D’Ippolito G, Diabira S, Howard GA et al. Marrow-isolated adult multilineage

inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential. J Cell Sci 2004;117:2971–2981.

Hess D, Li L, Martin M, Sakano S, Hill D, Strutt B, et al. Bone marrow-derived stem cell initiate pancreatic regeneration. Nat Biotechnol 2003; 21:763-770.

Tang DQ, Cao LZ, Burkhardt BR, Xia CQ, Litherland SA, Atkinson MA, et al. In vivo and in vitro characterization of insulin-producing cells obtained from murine bone marrow. Diabetes 2004; 53: 1721-1732.

Wang X, Willenbring H, Akkari Y, Torimaru Y, Foster M, Al-Dhalimy M, et al. Grompe, Cell fusion is the principal source of bone-marrow-derived hepatocytes. Nature 2003; 422:897-901.

Vassilopoulos G, Wang PR, Russell DW. Transplanted bone marrow regenerates liver by cell fusion. Nature 2003, 422:901-904.

Krause DS, Theise ND, Collector MI et al. Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell 2001;105:369–377.

Assady S, Maor G, Amit M et al. Insulin production by human embryonic stem cells. Diabetes 2001;50:1691–1697.

Castaing M, Peault B, Basmaciogullari A et al. Blood glucose normalization upon transplantation of human embryonic pancreas into beta-celldeficient SCID mice. Diabetologia 2001;44:2066–2076.

Soria B, Roche E, Berna G et al. Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice. Diabetes 2000;49:157–162.

Colter DC, Class R, DiGirolamo CM et al. Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc Natl Acad Sci U S A 2000;97:3213–3218.

Friedenstein AJ, Gorskaja JF, Kulagina NN. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 1976;4:267–274.

Schwarz EJ, Alexander GM, Prockop DJ et al. Multipotential marrow stromal cells transduced to produce L-DOPA: engraftment in a rat model of Parkinson disease. Hum Gene Ther 1999;10:2539–2549.

Lee K, Majumdar MK, Buyaner D et al. Human mesenchymal stem cells maintain transgene expression during expansion and differentiation. Mol Ther 2001;3:857–866.

Allay JA, Dennis JE, Haynesworth SE et al. LacZ and interleukin-3 expression in vivo after retroviral transduction of marrow-derived human osteogenic mesenchymal progenitors. Hum Gene Ther 1997;8:1417–1427.

Bhatia, S. N., Balis, U. J., Yarmush, M. L., and Toner, M. (1999) Effect of cell-cell interactions in preservation of cellular phenotype: Cocultivation of hepatocytes and nonparenchymal cells. FASEB J. 13, 1883–1900.

Guo Z, Yang J, Liu X, Li X, Hou C, Tang PH, et al. Biological features of mesenchymal stem cells from human bone marrow. Chin Med J 2001; 114:950-953.

Zhang Y, Li CD, Jiang XX, Li HL, Tang PH, Mao N. Comparison of mesenchymal stem cells from human placenta and bone marrow. Chin Med J 2004; 117:882-887.




DOI: http://dx.doi.org/10.52155/ijpsat.v21.2.2000

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Mahmoud Elhussiny Salama

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.