Resistance Of Antibiotic In Klebsiella Sp Isolate Of Chicken Origin And Feed Formulation

Rahmat Hidayat, Fachriyan Hasmi Pasaribu, Sri Mulatsih, Akhmad Arif Amin

Abstract


Antibiotics are one of the best achievements of modern medicine due to their importance in medical practice. However, antibiotics are currently considered less effective due to the emergence of antibiotic resistance worldwide. This study aims to identify Klebsiella sp resistance to 14 types of antibiotics and to produce feeds containing immunoglobulin Y(Ig Y) against Klebsiella sp. A total of 400 samples were isolated from Regencies, Cianjur, Sukabumi, and Bogor identified through a series of culturing, staining, and biochemical tests. Of the 400 isolated samples tested, 40 Klebsiella isolates were isolated. The result of the resistance test for these 40 Klebsiella sp isolates showed different results, with only 2 Klebsiella sp isolates showing no antibiotic resistance. Another result in the seventh week after vaccination was the presence of positive anti-Klebsiella sp. The provocation test after feeding the Ig Y formula did not cause death in chickens. The group of chickens fed either a 10% or 20% Ig Y diet, showed normal behavior, were healthy, and had a good appetite and dry stools. In comparison, the chickens that did not receive the Ig Y feed showed abnormal behavior, less agility, lack of appetite, and wet stools. The results of the clearance test showed that 8 treated chickens with resistant isolate samples obtained a good score, while in the group of sensitive isolate samples, 2 out of 8 treated chickens did not obtain a good score. The result of cross-reaction Ig Y against Klebsiella sp test to E. coli and Salmonella sp was negative.


Keywords


Antibiotics, Klebsiella sp, Resistance, Immunoglobulin Y, Feed

Full Text:

PDF

References


Aarestrup F. 2012. Get pigs off antibiotics: Frank Aarestrup explains how he helped Denmark to cut the use of antibiotics in its livestock by 60% and calls on the rest of the world to follow suit. Nature. 486(7404):465–467.

[CLSI] Clinical and Laboratory Standards Institute. 2020. Performance Standards for Antimicrobial Susceptibility Testing. 30th Edition. Wayne: Clinical and Laboratory Standards Institute.

Davis GS, Waits K, Nordstrom L, Grande H, Weaver B, Papp K, Horwinski J, Koch B, Hungate BA, Liu CM, et al. 2018. Antibiotic-resistant Escherichia coli from retail poultry meat with different antibiotic use claims. BMC Microbiol 18(174): 1–7.

[Dirjenak dan Keswan] Direktorat Jenderal Peternakan dan Kesehatan Hewan. 2019. Statistik Peternakan dan Kesehatan Hewan 2019. Direktorat Jenderal Peternakan dan Kesehatan Hewan. Kementerian Pertanian Republik Indonesia

Hidayat R. 2006. Studi Protektivitas Imunoglobulin Y (Ig-Y) ANTI Aeromonas hydrophila pada ikan Mas (Cyprinus carpio L.) dan Gurame (Osphronemus gouramy L.). Tesis. Sekolah Pascasarjana IPB. Bogor.

Hidayat R. 2009. Prospek telur ayam berkhasiat imunoterapi terhadap infeksi Enterobacter sakazakii pada Balita. Panduan Seminar LPPM IPB. Bogor.

Indrawati A, R. Hidayat. 2009. Telur Ayam 3 in one Anti Flu Burung dan Anti Diare: Produksi, Efikasi dan Aplikasinya. Makalah Seminar

Hasil-Hasil Penelitian IPB Tahun 2009.

Larsson A, Balow RM, Lindahl TL, Forsberg PO. 1993. Chicken Antibodies Taking Advantage of Evolution. A Review. Poultry Science 72: 1807 – 1812.

Leslie GA, Clem LW. 1969. Phylogeny of Immunoglobin Structure and Function. III. Immunoglobulins of The Chicken. J. Exp.Med. 130, 1337-1352.

Logan LK, Weinstein RA. 2017. The epidemiology of carbapenem-resistant Enterobacteriaceae: the impact and evolution of a global menace. J Infect Dis. 15(215): S28–36.

Manyi-Loh C, Mamphweli S, Meyer E, Okoh A. 2018. Antibiotic use in agriculture and its consequential resistance in environmental sources: potential public health implications. Molecules. 23(795):1–48.

Markey B, Finola L, Marie A, Ann C, Dores M. 2013. Clinical Veterinary Microbiology Second Edition. China (CN): Elsevier.

Murphy D, Ricci A, Auce Z, Beechinor JB, Bergendahl H, Breathnach R, Bures J, Da Silva JPD, Hederova J. 2016. EMA and EFSA joint scientific opinion on measures to reduce the need to use antimicrobial agents in animal husbandry in the European Union, and the resulting impacts on food safety (RONAFA). EFSA Journal. 15(1):4666

Murwani S, Qosimah D, Amri IA. 2017. Penyakit Bakterial pada Ternak Hewan

Besar dan Unggas. Malang (ID): UB Press

Pasaribu, FH. 2009. Penggunaan pelet yang mengandung imunoglobulin Y (Ig Y) anti Koi Herpes Virus (KHV) dalam upaya pengendalian wabah penyakit KHV pada ikan Mas (Cyprinus carpio carpio) dan Koi (Cyprinus carpio koi). Makalah Seminar Hasil-Hasil Penelitian IPB Tahun 2009.

Rawendra R. 2005. Imunoglobulin Y (Ig Y) fraksi larut air (WSF) kuning telur kering beku anti Enteropathogenic Escherichia coli (EPEC). Institut Pertanian Bogor.

[WHO] World Health Organization. 2018. Critically important antimicrobials for human medicine, 6th Revision. Geneva: World Health Organization.

Wibawan, IWT., Retno D. S., Chandramaya S. D., Tiok B. T. 2003. Diktat Immunologi. Fakultas Kedokteran Hewan. IPB.




DOI: http://dx.doi.org/10.52155/ijpsat.v37.1.5072

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Rahmat Hidayat, Fachriyan Hasmi Pasaribu, Sri Mulatsih, Akhmad Arif Amin

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.