Antimicrobial Potential of Paenibacillus Polymyxa AALI Endophyte Isolated from Calotropis Procera

Tahany Mohamed Abd El-Rahman, Ahmed Atef El-Beih, Ahmed Mohamed Ali, Mohamed Gamal Salah

Abstract


The increased drug resistance spectrum of bacteria pathogens has become a major concern worldwide. The present study addresses the isolation and identification of potent endophytes associated with Calotropis procera. Based on 16S rRNA gene analysis, an endophyte strain AALI showing promising antibacterial activity was identified as Paenibacillus polymyxa. The cell-free supernatant was extracted with ethyl acetate and the crude extract exhibited feasible antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae. Herein, we highlighted the promising antimicrobial activity of P. polymyxa strain AALI endophyte and results suggest the potential use of its crude extract to combat bacterial pathogens, especially S. aureus.

Keywords


Antimicrobial, Endophyte, Paenibacillus, Screening

Full Text:

PDF

References


D. E. Okwu, “Evaluation of the chemical composition of medicinal plants belonging to Euphorbiaceae,” Pak Vet J, vol. 14, pp. 160–162, 2001.

H. O. Edeoga, D. E. Okwu, and B. O. Mbaebie, “Phytochemical constituents of some Nigerian medicinal plants,” African J. Biotechnol., vol. 4, no. 7, pp. 685–688, 2005.

E. Grondin et al., “A comparative study on the potential of epiphytic yeasts isolated from tropical fruits to produce flavoring compounds,” Int. J. Food Microbiol., vol. 203, pp. 101–108, 2015.

D. Biswas, P. Biswas, S. Nandy, A. Mukherjee, D. K. Pandey, and A. Dey, “Endophytes producing podophyllotoxin from Podophyllum sp. and other plants: A review on isolation, extraction and bottlenecks,” South African J. Bot., 2020.

Z. Liu, J. Zhou, Y. Li, J. Wen, and R. Wang, “Bacterial endophytes from Lycoris radiata promote the accumulation of Amaryllidaceae alkaloids,” Microbiol. Res., p. 126501, May 2020.

M. A. Abdalla et al., “Isolation of endophytic fungi from South African plants, and screening for their antimicrobial and extracellular enzymatic activities and presence of type I polyketide synthases,” South African J. Bot., 2020.

P. K. Sahu, S. Singh, A. Gupta, U. B. Singh, G. P. Brahmaprakash, and A. K. Saxena, “Antagonistic potential of bacterial endophytes and induction of systemic resistance against collar rot pathogen Sclerotium rolfsii in tomato,” Biol. Control, vol. 137, Oct. 2019.

J. B. Jouda et al., “Anti-mycobacterial activity of polyketides from Penicillium sp. endophyte isolated from Garcinia nobilis against Mycobacterium smegmatis,” Int. J. Mycobacteriology, vol. 5, no. 2, pp. 192–196, 2016.

A. Alvin, K. I. Miller, and B. A. Neilan, “Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds,” Microbiol. Res., vol. 169, no. 7–8, pp. 483–495, 2014.

A. A. L. Gunatilaka, “Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence,” J. Nat. Prod., vol. 69, no. 3, pp. 509–526, 2006.

D. W. Zhang et al., “A new polyketide synthase-nonribosomal peptide synthetase hybrid metabolite from plant endophytic fungus Periconia sp.,” Chinese Chem. Lett., vol. 27, no. 5, pp. 640–642, May 2016.

F. M. W. Nongkhlaw and S. R. Joshi, “Horizontal Gene Transfer of the Non-ribosomal Peptide Synthetase Gene Among Endophytic and Epiphytic Bacteria Associated with Ethnomedicinal Plants,” Curr. Microbiol., vol. 72, no. 1, pp. 1–11, Jan. 2016.

T. L. Nascimento, Y. Oki, D. M. M. Lima, J. S. Almeida-Cortez, G. W. Fernandes, and C. M. Souza-Motta, “Biodiversity of endophytic fungi in different leaf ages of Calotropis procera and their antimicrobial activity,” Fungal Ecol., vol. 14, pp. 79–86, Apr. 2015.

O. R. Adeoyo, B. I. Pletschke, and J. F. Dames, “Molecular identification and antibacterial properties of an ericoid associated mycorrhizal fungus,” BMC Microbiol., vol. 19, no. 1, p. 178, Aug. 2019.

M. R. Asadi Karam, M. Habibi, and S. Bouzari, “Urinary tract infection: Pathogenicity, antibiotic resistance and development of effective vaccines against Uropathogenic Escherichia coli,” Mol. Immunol., vol. 108, pp. 56–67, Apr. 2019.

H. Yu et al., “Recent developments and future prospects of antimicrobial metabolites produced by endophytes,” Microbiological Research, vol. 165, no. 6. Urban & Fischer, pp. 437–449, 20-Aug-2010.

E. N. Grady, J. MacDonald, L. Liu, A. Richman, and Z. C. Yuan, “Current knowledge and perspectives of Paenibacillus: A review,” Microbial Cell Factories, vol. 15, no. 1. BioMed Central Ltd., 01-Dec-2016.

H. Jeong, S. K. Choi, C. M. Ryu, and S. H. Park, “Chronicle of a soil bacterium: Paenibacillus polymyxa E681 as a Tiny Guardian of Plant and Human Health,” Frontiers in Microbiology, vol. 10, no. MAR. Frontiers Media S.A., 2019.

Z. He, D. Kisla, L. Zhang, C. Yuan, K. B. Green-Church, and A. E. Yousef, “Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin,” Appl. Environ. Microbiol., vol. 73, no. 1, pp. 168–178, Jan. 2007.

H. Liu et al., “Complete genome sequence of Paenibacillus polymyxa YC0573, a plant growth-promoting rhizobacterium with antimicrobial activity,” Genome Announc., vol. 5, no. 6, 2017.

E. Huang and A. E. Yousef, “Biosynthesis of paenibacillin, a lantibiotic with N-terminal acetylation, by Paenibacillus polymyxa,” Microbiol. Res., vol. 181, pp. 15–21, Dec. 2015.

Y. Deng, Z. Lu, H. Bi, F. Lu, C. Zhang, and X. Bie, “Isolation and characterization of peptide antibiotics LI-F04 and polymyxin B 6 produced by Paenibacillus polymyxa strain JSa-9,” Peptides, vol. 32, no. 9, pp. 1917–1923, Sep. 2011.




DOI: http://dx.doi.org/10.52155/ijpsat.v20.2.1833

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Mohamed Gamal Salah

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.